Plant Cell Reports

, Volume 12, Issue 6, pp 324–327

A modified method for routine Agrobacterium-mediated transformation of in vitro grown potato microtubers

Authors

  • Gordon W. Snyder
    • Western Regional Research Center, U.S.Department of AgricultureAgricultural Research Service
  • William R. Belknap
    • Western Regional Research Center, U.S.Department of AgricultureAgricultural Research Service
Article

DOI: 10.1007/BF00237428

Cite this article as:
Snyder, G.W. & Belknap, W.R. Plant Cell Reports (1993) 12: 324. doi:10.1007/BF00237428

Summary

In vitro-grown potato (Solanum tuberosum L.) microtubers were used as an explant source in the production of transgenic plants by Agrobacterium-mediated gene transfer. In this study we tested four diverse potato cultivars, Lemhi Russet, Russet Burbank, Wauseon, and Yankee Chipper on various levels of zeatin riboside and 3-indoleacetyl-DL-aspartic acid for their ability to regenerate transgenic plants after infection with Agrobacterium tumefaciens. Culturing microtuber blocks from the medullary area separately from cortex and epidermal tissue containing the eyes resulted in fewer transgenic plants, with transgenic shoots arising only from the tissue with the eyes. Lemhi and Russet Burbank microtuber discs were also transformed with a chimeric gene, CLaSP, designed to increase resistance to blackspot bruise in the tuber. This method resulted in transformed plants in every experiment, with an efficiency that appeared to be genotype dependent.

Abbreviations

GUS

β-glucuronida (uidA)

IAA-AA

3-indoleacetyl-DL-aspartic acid

LB

Luria-Bertani

LSP

larval serum storage protein

nos

nopaline synthase

npt II

neomycin phosphotransferase

MS

Murashige and Skoog

PHA

phytohemaglutinin

ZR

zeatin riboside

Copyright information

© Springer-Verlag 1993