Article

Plant Cell Reports

, Volume 11, Issue 5, pp 238-242

First online:

Agrobacterium-mediated transformation of Citrus stem segments and regeneration of transgenic plants

  • G. A. MooreAffiliated withFruit Crops Department, Institute of Food and Agricultural Sciences, University of Florida
  • , C. C. JaconoAffiliated withFruit Crops Department, Institute of Food and Agricultural Sciences, University of Florida
  • , J. L. NeidighAffiliated withFruit Crops Department, Institute of Food and Agricultural Sciences, University of Florida
  • , S. D. LawrenceAffiliated withFruit Crops Department, Institute of Food and Agricultural Sciences, University of Florida
  • , K. ClineAffiliated withFruit Crops Department, Institute of Food and Agricultural Sciences, University of Florida

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Summary

A method for Agrobacterium-mediated transformation of Citrus and organogenic regeneration of transgenic plants is reported. Internodal stem segments were co-cultured with Agrobacterium harboring binary vectors that contained the genes for the scorable marker ß-glucuronidase (GUS) and the selectable marker NPT-II. A low but significant percentage (≤ 5%) of the shoots regenerated in the presence of 100 μg/ml kanamycin were GUS+. Polymerase chain reaction (PCR) analysis confirmed that GUS+ shoots contained T-DNA. Two plants established in soil were shown to be transgenic by Southern analysis.

Key words

Carrizo citrange Key lime (C. aurantifolia) Genetic transformation