Plant Cell Reports

, Volume 11, Issue 11, pp 576–580

Genotype-specific heat shock proteins in two maize inbreds

  • Julie A. Jorgensen
  • Jian Weng
  • Tuan-hua David Ho
  • Henry T. Nguyen
Article

DOI: 10.1007/BF00233096

Cite this article as:
Jorgensen, J.A., Weng, J., Ho, T.D. et al. Plant Cell Reports (1992) 11: 576. doi:10.1007/BF00233096
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Summary

Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indicated that Mol7 possesses greater potential than B73 to acquire thermal tolerance. Poly(A)+ RNA, extracted from leaf blades, was translated in vitro in the presence of 35S-methionine and the translation products separated by twodimensional gel electrophoresis. Major genotypic differences were observed in the translation products. Mo 17 synthesized twelve unique heat shock proteins in the 15–18 kD range, but B73 synthesized only three unique heat shock proteins in the same range. DNA polymorphisms were observed between the maize lines using 32P labeled heat shock protein gene probes.

Abbreviations

HKT

Heat-killing time

HS

Heat shock

HSP

Heat shock protein

HMW

High molecular weight

LMW

Low molecular weight

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Julie A. Jorgensen
    • 1
  • Jian Weng
    • 1
  • Tuan-hua David Ho
    • 2
  • Henry T. Nguyen
    • 1
  1. 1.Plant Genetics Laboratory, Mail Stop 2122, Texas Tech UniversityLubbockUSA
  2. 2.Department of BiologyWashington UniversitySt. LouisUSA

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