Plant Cell Reports

, Volume 15, Issue 7, pp 516–521

A reliable method for extraction of RNA from various conifer tissues

Authors

  • Jin-Zhuo Dong
    • Plant Biotechnology Institute, National Research Council of Canada
  • David I. Dunstan
    • Plant Biotechnology Institute, National Research Council of Canada
Article

DOI: 10.1007/BF00232985

Cite this article as:
Dong, J. & Dunstan, D.I. Plant Cell Reports (1996) 15: 516. doi:10.1007/BF00232985

Summary

A simple and efficient procedure suitable for extraction of high-quality RNA from cultured conifer tissues, somatic embryos, zygotic embryos, needles, stem and root tissues was developed. It produced from 100 μg up to 700 μg total RNA per gram tissue dependent on the types of tissues used. RNA quality was estimated by spectrophotometry, agarose gel electrophoresis, in vitro translation of mRNA, cDNA synthesis and Northern blot analysis. The method also worked well with Arabidopsis thaliana and tobacco tissues.

Key words

coniferPicea glaucaRNA extraction

Abbreviations

CTAB

cetyltrimethylammonium bromide

DEPC

diethylpyrocarbonate

PVP

polyvinylpyrrolidone

SDS-PAGE

sodium dodecyl sulphate-polyacrylamide gel electrophoresis

Copyright information

© Springer-Verlag 1996