Plant Cell Reports

, Volume 12, Issue 11, pp 644-647

First online:

Factors influencing transformation frequency of tomato (Lycopersicon esculentum)

  • Jeroen S. C. van RoekelAffiliated withMOGEN International NV
  • , Brigitte DammAffiliated withMOGEN International NV
  • , Leo S. MelchersAffiliated withMOGEN International NV
  • , André HoekemaAffiliated withMOGEN International NV

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


We developed an efficient procedure for transformation and regeneration of L. esculentum cv. Moneymaker from cotyledon explants. The effect of two parameters on the transformation frequency was investigated in detail. The use of feeder layers during cocultivation proved to be critical. In addition, it was found that Agrobacterium strains harbouring a L,L-succinamopine type helper plasmid yielded significantly higher transformation frequencies than those with octopine or nopaline type helper plasmids. The optimized protocol was used to obtain transformation frequencies averaging 9%. Of the plants produced approximately 80% proved to be diploid, of which 67% contained the transgene(s) on a single locus.