Summary
Transgenic sweet orange (Citrus sinensis L. Osbeck) plants have been obtained by Agrobacterium tumefaciens-mediated gene transfer. An hypervirulent A. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase II (NPT II) and ß-glucuronidase (GUS) genes was cocultivated with stem segments from in vivo grown seedlings. Shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. Shoot basal portions were assayed for GUS activity and the remaining portions were shoot tip grafted in vitro for production of plants. Integration of the GUS gene was confirmed by Southern analysis. This transformation procedure showed the highest transgenic plant production efficiency reported for Citrus.
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Abbreviations
- BA:
-
benzyladenine
- CaMV:
-
cauliflowermosaic virus
- GUS:
-
ß-glucuronidase
- LB:
-
Luria Broth
- MS:
-
Murashige and Skoog
- NAA:
-
naphthalenacetic acid
- NOS:
-
nopaline synthase
- NPT II:
-
neomycin phosphotransferase II
- PEG:
-
polyethylene glycol
- RM:
-
rooting medium
- SRM:
-
shoot regeneration medium
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Communicated by I. Potrykus
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Peña, L., Cervera, M., Juárez, J. et al. Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants. Plant Cell Reports 14, 616–619 (1995). https://doi.org/10.1007/BF00232724
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DOI: https://doi.org/10.1007/BF00232724