Plant Cell Reports

, Volume 11, Issue 3, pp 137–141

Transgenic poplars: expression of chimeric genes using four different constructs

  • Jean Charles Leple
  • Ana Cristina Miranda Brasileiro
  • Marie France Michel
  • Francis Delmotte
  • Lise Jouanin
Article

DOI: 10.1007/BF00232166

Cite this article as:
Leple, J.C., Brasileiro, A.C.M., Michel, M.F. et al. Plant Cell Reports (1992) 11: 137. doi:10.1007/BF00232166

Summary

Leaf or stem explants of a hybrid poplar clone (Populus tremula X Populus alba), sensitive to Agrobacterium tumefaciens, were co-cultivated either by an octopine or a nopaline disarmed A. tumefaciens modified strain. Transformed poplar shoots were readily regenerated from explants. The protocol was improved using the nopaline disarmed strain C58/pMP90 with the binary vector pBI121. This protocol was then used to test three other vectors. The first one, possessing a nptII gene fused to the CaMV 19S promoter, permitted regeneration of transformed shoots in presence of 50 to 100 mg/l kanamycin. The two other vectors carried an additional nptII gene under the control of the CaMV 35S or CaMV 35S promoter with a double enhancer sequence (CaMV 70). CaMV 70 promoter provided consistently higher level of gene expression than the other promoters in both callus and leaf tissues.

Abbreviations

CaMV

Cauliflower Mosaïc Virus

2iP

2-isopentenyladenine

GUS and gus

ß-glucuronidase

NAA

1-naphthaleneacetic acid

NPTII and nptII

neomycin phosphotransferase II

NOS

Nopaline synthase, X-Gluc: 5-bromo-4-chloro-3-indolyl ß-D glucuronide

Ap

ampicillin

Gn

gentamycin

Km

kanamycin

Rf

rifampicin

St

streptomycin

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Jean Charles Leple
    • 1
  • Ana Cristina Miranda Brasileiro
    • 2
  • Marie France Michel
  • Francis Delmotte
    • 3
  • Lise Jouanin
    • 2
  1. 1.Station d'Amélioration des Arbres Forestiers, Institut National de la Recherche AgronomiqueArdonFrance
  2. 2.Laboratoire de Biologie Cellulaire, Institut National de la Recherche AgronomiqueVersailles CedexFrance
  3. 3.Centre de Biophysique Moléculaire, Département de Biochimie des Glycoconjugués et Lectines EndogènesCentre National de la Recherche Scientifique et Université d'OrléansOrléans Cedex 2France

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