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Agrobacterium tumefaciens-mediated transformation of Artemisia annua L. and regeneration of transgenic plants

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Summary

A transformation system was developed for Artemisia annua L. plants. Leaf explants from in vitro grown plants developed callus and shoots on medium with 0.05 mg/L naphthaleneacetic acid and 0.5 mg/L N6-benzyladenine after transformation with the C58C1 RifR (pGV2260) (pTJK136) Agrobacterium tumefaciens strain. A concentration of 20 mg/L kanamycin was added in order to select transformed tissue. Kanamycin resistant shoots were rooted on naphthaleneacetic acid 0.1 mg/L. Polymerase chain reactions and DNA sequencing of the amplification products revealed that 75% of the regenerants contained the foreign genes. 94% of the transgenic plants showed a β-glucuronidase-positive response.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

BA:

N6-benzyladenine

GM:

germination medium

GMVIT:

germination medium with vitamins

GUS:

β-glucuronidase

Kin:

kinetin (N6-furfurylaminopurine)

NAA:

α-naphthaleneacetic acid

NPT II:

neomycin phosphotransferase II

PCR:

Polymerase Chain Reaction

T-DNA:

transfer-DNA

X-glucuronide:

5-bromo-4-chloro-3-indolyl β-D-glucuronide

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Authors and Affiliations

  1. Laboratorium voor Farmaceutische Biotechnologie, Universiteit Gent, Harelbekestraat 72, B-9000, Gent, Belgium

    Annemieke Vergauwe, Ronny Cammaert, Dirk Vandenberghe & Elfride Van den Eeckhout

  2. Laboratorium voor Genetica, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000, Gent, Belgium

    Christiane Genetello, Dirk Inze & Marc Van Montagu

Authors
  1. Annemieke Vergauwe
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  2. Ronny Cammaert
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  3. Dirk Vandenberghe
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  4. Christiane Genetello
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  5. Dirk Inze
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  6. Marc Van Montagu
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  7. Elfride Van den Eeckhout
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Communicated by W. Barz

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Vergauwe, A., Cammaert, R., Vandenberghe, D. et al. Agrobacterium tumefaciens-mediated transformation of Artemisia annua L. and regeneration of transgenic plants. Plant Cell Reports 15, 929–933 (1996). https://doi.org/10.1007/BF00231590

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  • DOI: https://doi.org/10.1007/BF00231590

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