Plant Cell Reports

, Volume 9, Issue 2, pp 65–68

Production of theanine and other γ-glutamyl derivatives by Camellia sinensis cultured cells

  • Yutaka Orihara
  • Tsutomu Furuya

DOI: 10.1007/BF00231550

Cite this article as:
Orihara, Y. & Furuya, T. Plant Cell Reports (1990) 9: 65. doi:10.1007/BF00231550


The maximum theanine production by Camellia sinensis cultured cells was achieved by culturing in the modified MS medium containing 2 mg/l indole-3-butyric acid, 0.1 mg/l kinetin, 0 mM NH4NO3 and 39.6 mM KNO3 with 40 mM ethylamine hydrochloride or 20 mM ethylamine hydrochloride and 10 mM L-glutamic acid. Other primary amines, such as methylamine, n-butylamine, 2-hydroxyethylamine, 2cyanoethylamine, aniline, benzylamine and phenylethylamine, were also biotransformed to N5-alkyl-L-glutamine derivatives by C. sinensis cultured cells.



indole-3-butyric acid



MS medium

Murashige and Skoog's basal medium (1962)


nuclear magnetic resonance

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Yutaka Orihara
    • 1
  • Tsutomu Furuya
    • 1
  1. 1.School of Pharmaceutical Sciences, Kitasato UniversityTokyoJapan