Molecular and Cellular Biochemistry

, Volume 111, Issue 1, pp 33–40

Characterization of oxygen free radicals generated during vanadate-stimulated NADH oxidation


  • P. Kalyani
    • Department of BiochemistryIndian Institute of Science
  • S. Vijaya
    • Department of BiochemistryIndian Institute of Science
  • T. Ramasarma
    • Department of BiochemistryIndian Institute of Science

DOI: 10.1007/BF00229571

Cite this article as:
Kalyani, P., Vijaya, S. & Ramasarma, T. Mol Cell Biochem (1992) 111: 33. doi:10.1007/BF00229571


The oxidation of NADH and accompanying reduction of oxygen to H2O2 stimulated by polyvanadate was markedly inhibited by SOD and cytochrome c. The presence of decavanadate, the polymeric form, is necessary for obtaining the microsomal enzyme-catalyzed activity. The accompanying activity of reduction of cytochrome c was found to be SOD-insensitive and therefore does not represent superoxide formation. The reduction of cytochrome c by vanadyl sulfate was also SOD-insensitive. In the presence of H2O2 all the forms of vanadate were able to oxidize reduced cytochrome c, which was sensitive to mannitol, tris and also catalase, indicating H202-dependent generation of hydroxyl radicals. Using ESR and spin trapping technique only hydroxyl radicals, but not superoxide anion radicals, were detected during polyvanadate-dependent NADH oxidation.

Key words

vanadate stimulationNADH oxidationhydroxyl radicalssuperoxide formationSOD-insensitive cytochrome c reduction

Copyright information

© Kluwer Academic Publishers 1992