Applied Microbiology and Biotechnology

, Volume 39, Issue 2, pp 197–203

Cloning and sequence analysis of the cyclomaltodextrinase gene from Bacillus sphaericus and expression in Escherichia coli cells


  • Tetsuya Oguma
    • Noda Institute for Scientific Research
  • Asahi Matsuyama
    • Noda Institute for Scientific Research
  • Mamoru Kikuchi
    • Research and Development DivisionKikkoman Corporation
  • Eiichi Nakano
    • Research and Development DivisionKikkoman Corporation

DOI: 10.1007/BF00228606

Cite this article as:
Oguma, T., Matsuyama, A., Kikuchi, M. et al. Appl Microbiol Biotechnol (1993) 39: 197. doi:10.1007/BF00228606


The gene for cyclomaltodextrinase (CDase; EC from Bacillus sphaericus E-244 was cloned in the recombinant plasmid pCD629. Sequencing a portion of pCD629 revealed a unique open reading frame of 1,773 nucleotides coding for a 591-amino-acid polypeptide. The deduced polypeptide sequence showed about 50% homology with that of a neopullulanase, and was slightly homologous to those of the cyclodextrin glucanotransferases and the α-amylases. The optimum pH, specific activity and Km value for β-cyclodextrin of the CDase that has been produced in Escherichia coli cells were 8.0, 16.4 units/mg protein, and 0.41 mm, respectively. These values were almost identical to those from B. sphaericus E-244.

Copyright information

© springer-Verlag 1993