Molecular and Cellular Biochemistry

, Volume 91, Issue 1, pp 39–44

Membrane effects of the polyene antibiotic amphotericin B and of some of its derivatives on lymphocytes

Authors

  • N. Henry-Toulmé
    • Laboratoire de Physique et Chimie BiomoléculaireCNRS USA 198
  • P. Sarthou
    • Unité d'Immunochimie Analytique, Départment d'ImmunologieInstitut Pasteur
  • M. Seman
    • Laboratoire d'ImmunodifférenciationInstitut Jacques Monod
  • J. Bolard
    • Laboratoire de Physique et Chimie BiomoléculaireCNRS USA 198
Invited Paper

DOI: 10.1007/BF00228077

Cite this article as:
Henry-Toulmé, N., Sarthou, P., Seman, M. et al. Mol Cell Biochem (1989) 91: 39. doi:10.1007/BF00228077

Abstract

Amphotericin B (AmB) exhibits immunomodulating properties in mice.In vitro studies on lymphocytes, in relation with these properties, are reported here with AmB and two of its derivatives: the N-Fructosyl (N-Fru AmB) and the N-thiopropionyl (AmBSH) derivatives. Interactions of these molecules with thymocytes, a sensitive cell type, demonstrated that the extent of binding is not a toxicity parameter. In contrast, membrane fludity changes have been observed and appeared to be related to toxicity.

Experiments performed with normal B lymphocytes have shown that Amphotericin B derivatives were more potent polyclonal B cell activators than the parent compound. To go further in the understanding of these events, we have investigated in a B cell line WEHI 231, the changes in intracellular Ca2+ and membrane potential induced by AmB and AmBSH. The two polyenes were shown to induce membrane depolarization but no intracellular Ca2+ increase.

Key words

polyene antibioticsamphotericin Bimmunomodulationmembrane interactionsmembrane potentialintracellular Ca2+

Copyright information

© Kluwer Academic Publishers 1989