Acta Neuropathologica

, Volume 84, Issue 3, pp 265–272

Monoclonal antibodies with selective specificity for Alzheimer Tau are directed against phosphatase-sensitive epitopes

Authors

  • Marc Mercken
    • Innogenetics, Industriepark Zwijnaarde
    • Laboratorie of Neurobiology Born-Bunge FoundationUniversity of Antwerp (UIA)
  • Marc Vandermeeren
    • Innogenetics, Industriepark Zwijnaarde
    • Laboratorie of Neurobiology Born-Bunge FoundationUniversity of Antwerp (UIA)
  • Ursula Lübke
    • Laboratorie of Neuropathology, Born-Bunge FoundationUniversity of Antwerp (UIA)
  • Jan Six
    • Innogenetics, Industriepark Zwijnaarde
    • Laboratorie of Neurobiology Born-Bunge FoundationUniversity of Antwerp (UIA)
  • Jef Boons
    • Laboratorie of Neurobiology Born-Bunge FoundationUniversity of Antwerp (UIA)
  • André Van de Voorde
    • Innogenetics, Industriepark Zwijnaarde
  • Jean-Jacques Martin
    • Laboratorie of Neuropathology, Born-Bunge FoundationUniversity of Antwerp (UIA)
  • Jan Gheuens
    • Laboratorie of Neurobiology Born-Bunge FoundationUniversity of Antwerp (UIA)
Regular Papers

DOI: 10.1007/BF00227819

Cite this article as:
Mercken, M., Vandermeeren, M., Lübke, U. et al. Acta Neuropathol (1992) 84: 265. doi:10.1007/BF00227819

Summary

A modified form of the microtubule-associated protein Tau is the major component of the paired helical filaments (PHF) found in Alzheimer's disease. The characterization of these posttranslational Tau modifications is hindered by the lack of sufficient PHF-Tau-specific markers. Here we describe several monoclonal antibodies, prepared by immunization with PHF, two of which showed a selective specificity for PHF-Tau without cross-reactivity with normal Tau. Epitope recognition by these two monoclonals was sensitive to alkaline phosphatase treatment. In Western blotting these monoclonal antibodies reacted specifically with the abnormally phosphorylated epitopes on Alzheimer's disease-associated PHF-Tau. One of the new antibodies can be used for the construction of a sandwich enzyme-linked immunosorbent assay for the specific detection of PHF-Tau without cross-reactivity to normal Tau proteins.

Key words

Alzheimer's diseaseTauPhosphorylationMonoclonal antibodies
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Copyright information

© Springer-Verlag 1992