Molecular and Cellular Biochemistry

, Volume 105, Issue 2, pp 107–117

Uteroferrin contains complex and high mannose-type oligosaccharides when synthesized in vitro

  • George A. Baumbach
  • Philippa T. K. Saunders
  • Catherine M. Ketcham
  • Fuller W. Bazer
  • R. Michael Roberts

DOI: 10.1007/BF00227750

Cite this article as:
Baumbach, G.A., Saunders, P.T.K., Ketcham, C.M. et al. Mol Cell Biochem (1991) 105: 107. doi:10.1007/BF00227750


Mature uteroferrin (Uf; MΓ = 35,500) is a progesterone-induced acid phosphatase secreted by the pig uterus. It contains a single, unphosphorylated, high mannose-type oligosaccharide. Endometrial explants cultured in vitro secrete Uf with a MΓ of 37,000 (37k Uf) having phosphorylated high mannose oligosaccharides. In this report we demonstrate that 37k Uf contains two N-linked oligosaccharides which are a mixture of complex and high mannose-type oligosaccharides. The complex-type glycopeptides are biantennary and a portion may be fucosylated on the GlcNac of the chitobiose core proximal to the peptide. Only a portion of the high mannose-type oligosaccharides are phosphorylated. The remainder appear to be typical Man6-4GlcNac2 oligosaccharides found on mature Uf.

Key words

iron transportuteroferringlycoproteinpregnancyswine





Concanavalin A


Wheat Germ Agglutinin


endo-β-N-acetylglucosaminidase H


Sodium Dodecyl Sulfate


polyacrylamide gel electrophoresis in the presence of SDS

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • George A. Baumbach
    • 1
  • Philippa T. K. Saunders
    • 2
  • Catherine M. Ketcham
    • 2
  • Fuller W. Bazer
    • 3
  • R. Michael Roberts
    • 4
  1. 1.Department of Animal Science, College of Veterinary MedicineThe University of TennesseeKnoxvilleUSA
  2. 2.Department of Biochemistry and Molecular BiologyUniversity of FloridaGainesvilleUSA
  3. 3.Department of Animal ScienceUniversity of FloridaGainesvilleUSA
  4. 4.Department of Animal ScienceUniversity of MissouriColumbiaUSA
  5. 5.MRC Reproductive Biology UnitCentre for Reproductive BiologyEdinburghScotland
  6. 6.Division of Hematology, OncologyWashington University School of Medicine and BiochemistrySt LouisUSA