Article

Molecular and Cellular Biochemistry

, Volume 105, Issue 2, pp 107-117

Uteroferrin contains complex and high mannose-type oligosaccharides when synthesized in vitro

  • George A. BaumbachAffiliated withDepartment of Animal Science, College of Veterinary Medicine, The University of Tennessee
  • , Philippa T. K. SaundersAffiliated withDepartment of Biochemistry and Molecular Biology, University of Florida
  • , Catherine M. KetchamAffiliated withDepartment of Biochemistry and Molecular Biology, University of Florida
  • , Fuller W. BazerAffiliated withDepartment of Animal Science, University of Florida
  • , R. Michael RobertsAffiliated withDepartment of Animal Science, University of Missouri

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Abstract

Mature uteroferrin (Uf; MΓ = 35,500) is a progesterone-induced acid phosphatase secreted by the pig uterus. It contains a single, unphosphorylated, high mannose-type oligosaccharide. Endometrial explants cultured in vitro secrete Uf with a MΓ of 37,000 (37k Uf) having phosphorylated high mannose oligosaccharides. In this report we demonstrate that 37k Uf contains two N-linked oligosaccharides which are a mixture of complex and high mannose-type oligosaccharides. The complex-type glycopeptides are biantennary and a portion may be fucosylated on the GlcNac of the chitobiose core proximal to the peptide. Only a portion of the high mannose-type oligosaccharides are phosphorylated. The remainder appear to be typical Man6-4GlcNac2 oligosaccharides found on mature Uf.

Key words

iron transport uteroferrin glycoprotein pregnancy swine