Theoretical and Applied Genetics

, Volume 81, Issue 5, pp 581–588

pEmu: an improved promoter for gene expression in cereal cells

  • D. I. Last
  • R. I. S. Brettell
  • D. A. Chamberlain
  • A. M. Chaudhury
  • P. J. Larkin
  • E. L. Marsh
  • W. J. Peacock
  • E. S. Dennis
Originals

DOI: 10.1007/BF00226722

Cite this article as:
Last, D.I., Brettell, R.I.S., Chamberlain, D.A. et al. Theoret. Appl. Genetics (1991) 81: 581. doi:10.1007/BF00226722
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Summary

A recombinant promoter, pEmu, has been constructed to give a high level of gene expression in monocots. It is based on a truncated maize Adh1 promoter, with multiple copies of the Anaerobic Responsive Element from the maize Adh1 gene and ocs-elements from the octopine synthase gene of Agrobacterium tumefaciens. The pEmu promoter was one of 12 different promoter constructs that were linked to the β-glucuronidase (GUS) marker gene. Promoter activity was measured 48 h after introduction of the constructs into protoplasts of five different monocot species [wheat, maize, rice, einkorn (Triticum monococcum), and Lolium multiflorum] and one dicot (Nicotiana plumbaginifolia). In suspension cell protoplasts, the most highly expressing construct (pEmuGN) gave 10- to 50-fold higher expression than the CaMV 35S promoter in all the monocot species. The pEmu promoter should be valuable where a high level of gene expression is required in monocots. The pEmu promoter showed instability in several widely used Escherichia coli strains but was stable in a recA, recD strain AC001, which is described. Another construct, p4OCSΔ35SIGN, gave a tenfold increase in expression over the CaMV 35S promoter in dicot (Nicotiana plumbaginifolia) protoplasts.

Key words

Promoter Enhancers Monocot Electroporation Plant transformation 

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • D. I. Last
    • 1
  • R. I. S. Brettell
    • 1
  • D. A. Chamberlain
    • 1
  • A. M. Chaudhury
    • 1
  • P. J. Larkin
    • 1
  • E. L. Marsh
    • 1
  • W. J. Peacock
    • 1
  • E. S. Dennis
    • 1
  1. 1.Division of Plant IndustryC.S.I.R.O.CanberraAustralia

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