Cell and Tissue Research

, Volume 153, Issue 1, pp 55–61

Vital staining of specific monoamine-containing cells in the leech nervous system

  • Ann E. Stuart
  • A. J. Hudspeth
  • Zach W. Hall
Article

DOI: 10.1007/BF00225445

Cite this article as:
Stuart, A.E., Hudspeth, A.J. & Hall, Z.W. Cell Tissue Res. (1974) 153: 55. doi:10.1007/BF00225445

Summary

Neutral red and several related dyes selectively stain certain cells in the ventral nerve cord of the leech. These cells are identical with those that can be shown by the FalckHillarp fluorescence technique to contain serotonin or a catecholamine; evidence suggests that the catecholamine is dopamine. Although the mechanism of staining remains unknown, it does not depend on active uptake of the dye. Stained cells in ganglia that were incubated for 12 hours in culture medium retained their normal physiological properties. Thus neutral red may be useful for locating specific monoamine-containing neurons in living nervous tissue.

Key words

Neutral red Leech Vital staining Serotonin Catecholamines 

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Ann E. Stuart
    • 1
  • A. J. Hudspeth
    • 1
  • Zach W. Hall
    • 1
  1. 1.Department of NeurobiologyHarvard Medical SchoolBostonUSA
  2. 2.Konung Gustav V:s forskningsinstitutStockholmSweden

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