Theoretical and Applied Genetics

, Volume 80, Issue 2, pp 209–222

Chloroplast DNA diversity in wild and cultivated species of rice (Genus Oryza, section Oryza). Cladistic-mutation and genetic-distance analysis

  • A. M. Dally
  • G. Second

DOI: 10.1007/BF00224389

Cite this article as:
Dally, A.M. & Second, G. Theoret. Appl. Genetics (1990) 80: 209. doi:10.1007/BF00224389


Using a novel nonaqueous procedure, chloroplast DNA was isolated from 318 individual adult rice plants, representing 247 accessions and the breadth of the diversity in section Oryza of genus Oryza. Among them, 32 different cpDNA restriction patterns were distinguished using the restriction endonucleases EcoRI and AvaI, and they were further characterized by restriction with BamHI, HindIII, SmaI, PstI, and BstEII enzymes. The differences in the electrophoretic band patterns were parsimoniously interpreted as being the result of 110 mutations, including 47 restriction site mutations. The relationships between band patterns were studied by a cladistic analysis based on shared mutations and by the computation of genetic distances based on shared bands. The deduced relationships were compared with earlier taxonomical studies. The maternal parents for BC genome allotetraploids were deduced. Within species, cpDNA diversity was found larger in those species with an evolutionary history of recent introgression and/or allotetraploidization. Occasional paternal inheritance and recombination of cpDNA in rice was suggested.

Key words

Oryzarice cpDNA diversityCpDNA inheritanceAllotetraploid progenitorsCladistic mutation

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • A. M. Dally
    • 1
  • G. Second
    • 1
  1. 1.French Institute of Scientific Research for Development in Cooperation ORSTOMMontpellier CenterMontpellier CedexFrance
  2. 2.Institute for the Developmental and Molecular Biology of PlantsDüsseldorfFRG