Theoretical and Applied Genetics

, Volume 80, Issue 6, pp 852–857

The isolation and characterization of gibberellin-deficient mutants in tomato


  • M. Koornneef
    • Department of GeneticsAgricultural University
  • T. D. G. Bosma
    • Department of GeneticsAgricultural University
  • C. J. Hanhart
    • Department of GeneticsAgricultural University
  • J. H. van der Veen
    • Department of GeneticsAgricultural University
  • J. A. D. Zeevaart
    • MSU-DOE Plant Research Laboratory, Michigan State University

DOI: 10.1007/BF00224204

Cite this article as:
Koornneef, M., Bosma, T.D.G., Hanhart, C.J. et al. Theoret. Appl. Genetics (1990) 80: 852. doi:10.1007/BF00224204


In tomato, nine independent EMS-induced mutants representing recessive mutations at three different loci (gib-1, gib-2, and gib-3) were isolated. Six of these have an almost absolute gibberellin requirement for seed germination and elongation growth. In addition, the leaves are darker green, smaller, and changed in structure as compared to wild type. The three other mutants, which germinate without GA, are allelic to specific, nongerminating mutants and have less severe mutant characteristics. The respective loci are situated on three different chromosomes. The genes identified by these mutants control steps in gibberellin biosynthesis, as endogenous gibberellins are strongly reduced.

Key words

Lycopersicon esculentumGibberellinGerminationDwarf mutantsGene localization

Copyright information

© Springer-Verlag 1990