Theoretical and Applied Genetics

, Volume 80, Issue 6, pp 826–832

Assessment of the degree and the type of restriction fragment length polymorphism in barley (Hordeum vulgare)

  • A. Graner
  • H. Siedler
  • A. Jahoor
  • R. G. Herrmann
  • G. Wenzel
Originals

DOI: 10.1007/BF00224200

Cite this article as:
Graner, A., Siedler, H., Jahoor, A. et al. Theoret. Appl. Genetics (1990) 80: 826. doi:10.1007/BF00224200

Summary

In order to determine the extent of polymorphism in barley (Hordeum vulgare), DNA from 48 varieties was analyzed with 23 genomic, single-copy probes, distributed across all seven chromosomes. Upon hybridization to wheat-barley addition lines, the probes showed different degrees of homology compared to the wheat genome. Polymorphisms were detected in the barley genome at a frequency of 43% after digestion with EcoRI, BamHI, and HindIII. Subgroups of spring and winter barley and of two- and six-rowed types showed less diversity which, in most cases, was due to shifts in allelic frequencies. One probe (MWG1H504) hybridized to an EcoRI restriction fragment exclusively observed in winter barley. A comparison of six different restriction enzymes revealed clear differences with regard to their efficiency in detecting polymorphisms. The respective frequencies were between 13% (HindIII) and 37% (EcoRV). A significant correlation between the efficiency of a restriction enzyme and the mean fragment size detected by the different probes identified insertion/deletion events as the major factor causing polymorphism in barley.

Key words

BarleyRestriction fragment length polymorphismDNAWheat-barley addition linesChromosome assignment

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • A. Graner
    • 1
  • H. Siedler
    • 1
  • A. Jahoor
    • 2
  • R. G. Herrmann
    • 3
  • G. Wenzel
    • 1
  1. 1.Institute for Resistance Genetics of the Federal Biological Research Center for Agronomy and ForestryGrünbachGermany
  2. 2.Institute for Plant Breeding of the Technical University of MunichFreising-WeihenstephanGermany
  3. 3.Institute for Botany of the Ludwig-Maximilians-UniversityMunich 19Germany