Theoretical and Applied Genetics

, Volume 80, Issue 6, pp 753–761

Mitochondrial DNA polymorphisms of Malawian bean lines: further evidence for two major gene pools

  • M. M. Khairallah
  • M. W. Adams
  • B. B. Sears
Originals

DOI: 10.1007/BF00224188

Cite this article as:
Khairallah, M.M., Adams, M.W. & Sears, B.B. Theoret. Appl. Genetics (1990) 80: 753. doi:10.1007/BF00224188

Summary

Intraspecific mitochondrial DNA (mtDNA) diversity was determined in 23 Phaseolus vulgaris genotypes, and compared to previously observed variability of morphoagronomic characters and isozyme loci. Twenty of the lines were collected from Malawian landraces; the other three were pure-bred cultivars. The mtDNAs were digested with eight restriction endonucleases, revealing complex banding patterns. Southern hybridization using cosmid clones covering about 200-kb of the genome showed a considerable amount of uniformity of the mtDNA banding patterns. However, five restriction fragment length polymorphisms (RFLPs) were detected, dividing the bean lines into two groups corresponding to the previously known Mesoamerican and Andean gene pools of P. vulgaris. The cultivar “Mecosta” was separated from the rest of the lines by an additional RFLP. At least two out of the six RFLPs are believed to be due to base-pair mutation events. Our results provide the first evidence that the cytoplasms of the two major germ plasm pools of beans are distinct.

Key words

Phaseolus vulgarisMitochondrial RFLPsGene poolsGenetic diversityMalawi

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • M. M. Khairallah
    • 1
  • M. W. Adams
    • 1
  • B. B. Sears
    • 2
  1. 1.Department of Crop and Soil SciencesMichigan State UniversityEast LansingUSA
  2. 2.Department of Botany and Plant PathologyMichigan State UniversityEast LansingUSA