Anther-specific expression of the rolB gene of Agrobacterium rhizogenes increases IAA content in anthers and alters anther development and whole flower growth
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
The promoter activity of a 2.2-kb DNA fragment from the 5′ flanking sequence of the tap1 gene of snapdragon has been characterised in tobacco with the β-glucuronidase reporter gene. The tap1 promoter conferred to the reporter gene an expression that was limited to the early stages of anther development. Expression of the rolB gene of Agrobacterium rhizogenes under the control of the tap1 promoter impaired the development of tobacco flowers. Tobacco plants transgenic for the tap1-rolB chimeric gene showed altered anthers and a reduction in whole flower growth. Such growth alterations were correlated with an increase in free IAA content and a decrease in gibberellin activity present in anthers. Since the rolB gene codes for an indole β-glucosidase (Estruch et al. 1991), we interpret the phenotypic alterations to be a consequence of the increased content and activity of auxin in anthers. The perturbation of anther development would in turn affect gibberellin production and content, which is reflected in a reduced elongation of flowers.
- Bala R, Rao IVR, Ram MYH (1985) Influence of stamens, gibberellic acid and sucrose on corolla growth in Gladiolus. J Plant Physiol 122:87–92.
- Cardarelli M, Mariotti D, Pomponi M, Spanó L, Capone I, Costantino P (1987) Agrobacterium rhizogenes TDNA genes capable of inducing hairy root phenotype. Mol Gen Genet 209:475–480.
- Estruch JJ, Schell J, Spena A (1991) The protein encoded by the rolB plant oncogene hydrolyses indole glucosides. EMBO J 11:3125–3128.
- Gifford EM, Foster AS (1988) Morphology and evolution of vascular plants. Freeman and Company, New York.
- Horsch RB, Fry JE, Hoffman NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229–1231.
- Jacobsen SE, Olszewski NE (1991) Characterization on the anest in anther development associated with gibberellin deficiency of the gib-1 mutant of tomato. Plant Physiol 97:409–414.
- Koncz C, Schell J (1986) The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector. Mol Gen Genet 204:383–396.
- Jefferson R (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387–405.
- Marrè E (1946) Aspetti istofisiologici dell' azione dello stame sul pistillo. Boll Soc Ital Biol Sper 22:1208–1209.
- Maurel C, Barbier-Brygoo H, Brevet J, Spena A, Tempe J, Guern J (1990) In: Hennecke H, Verma DP (eds) Advances in molecular genetics of plant-microbe interactions, vol 1. Kluwer Academic Press, Dordrecht Boston London, pp 343–351.
- Murakami Y (1968) A new rice seedling test for gibberellins, microdrop method and its use for testing extracts of rice and morning glory. Bot Mag 81:33–43.
- Murakami Y (1973) The role of gibberellins in the growth of floral organs of Pharbitis nil. Plant Cell Physiol 14:91–102.
- Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497.
- Nacken WKF, Huijser P, Beltran JP, Saedler H, Sommer H (1991) Molecular characterization of two stamen-specific genes, tap1 and fill, that are expressed in the wild type but not in the deficiens mutant of Antirrhinum majus. Mol Gen Genet 229:129–136.
- Plack A (1957) Sexual dimorphism in Labiatae. Nature 180:1218–1219.
- Plack A (1958) Effect of gibberellic acid on corolla size. Nature 182:610.
- Schmülling T, Schell J, Spena A (1988) Single genes from Agrobacterium rhizogenes influence plant development. EMBO J 7:2621–2629.
- Shen WH, Petit A, Guern J, Tempe J (1988) Hairy-roots are more sensitive to auxin than normal roots. Proc Natl Acad Sci USA 85:3417–3421.
- Spena A, Schmülling T, Koncz C, Schell J (1987) Independent and synergistic activity of rolA, rolB and rolC loci in stimulating abnormal growth in plants. EMBO J 6:3891–3899.
- Südi J (1964) Induction of the formation of complexes between aspartic acid and indolyl-3-acetic acid or 1-naphtalene acetic acid by other carboxylic acids. Nature 201:1009–1010.
- Südi J (1966) Increases in the capacity of pea tissue to form acyl-aspartic acids specifically induced by auxins. New Phytol 65:9–21.
- Van Onckelen H, Prinsen E, Chriqui D, Tepfer M, De Greef J (1988) Endogenous auxin levels in normal and hairy root tobacco plants. Med Fac Landbouw Rijkuniv Gent 53:1683–1693.
- Venis MA (1964) Induction of enzymatic activity by indolyl-3- acetic acid and its dependence on synthesis of ribonucleic acid. Nature 202:900–901.
- Venis MA (1972) Auxin-induced conjugation system in peas. Plant Physiol 49:24–27.
- Weiss D, Halevy AH (1989) Stamens and gibberellin in the regulation of corolla pigmentation and growth in Petunia hybrida. Planta 179:89–96.
- White FF, Taylor BH, Huffman GA, Gordon MP, Nester EW (1985) Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes. J Bacteriol 164:33–44.
- Yamada T, Palm CJ, Brooks B, Kosuge T (1985) Nucleotide sequences of the Pseudomonas savastanoi indoleacetic acid genes show homology with Agrobacterium tumefaciens T-DNA. Proc Natl Acad Sci USA 82:6522–6526.
- Zanoni G (1941) Ricerche sull' accrescimento del fiore. Azione correlativa degli sporofilli. Ann Bot 22:46–66.
- Anther-specific expression of the rolB gene of Agrobacterium rhizogenes increases IAA content in anthers and alters anther development and whole flower growth
Theoretical and Applied Genetics
Volume 84, Issue 5-6 , pp 520-527
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Anther-specific expression
- rolB gene
- Increased IAA content
- Decreased gibberellin activity
- Altered flower growth
- Industry Sectors