Theoretical and Applied Genetics

, Volume 84, Issue 1, pp 6–9

Characterization of a rice (Oryza sativa L.) mutant deficient in the heme domain of nitrate reductase

Authors

  • H. Hasegawa
    • Research Institute for Advanced Science and Technology
  • T. Katagiri
    • Faculty of AgricultureKagawa University
  • S. Ida
    • Research Institute for Food ScienceKyoto University
  • O. Yatou
    • Institute of Radiation Breeding, NIAR
  • M. Ichii
    • Faculty of AgricultureKagawa University
Article

DOI: 10.1007/BF00223974

Cite this article as:
Hasegawa, H., Katagiri, T., Ida, S. et al. Theoret. Appl. Genetics (1992) 84: 6. doi:10.1007/BF00223974

Summary

Biochemical and genetical characterization of a rice nitrate reductase (NR)-deficient mutant, M819, which had been isolated as a chlorate-resistant mutant, was carried out. In M819, leaf NADH-NR activity was found to be about 10% of that of the wild-type cv ‘Norin 8’, while NADPH-NR activity was higher than that in the wild-type; FMNH2-NR and MV-NR activities were also 10% of those of the wild type; BPB-NR activity was higher than that of the wild type; and xanthine dehydrogenase activity was revealed to be present in both. These results suggest that the mutant line M819 lacks the functional heme domain of the NADH-NR polypeptide due to a point mutation or a small deletion within the coding region of the structural gene. Chlorate resistance in M819 was transmitted by a single recessive nuclear gene.

Key words

Oryza sativa L.Nitrate reductaseDeficient mutantChlorate resistanceHeme domain

Abbreviations

NR

Nitrate reductase

NiR

nitrite reductase

FMNH2

reduced flavin nucleotide

MV

reduced methyl viologen

BPB

reduced bromphenol blue

XDH

xanthine dehydrogenase

Copyright information

© Springer-Verlag 1992