Theoretical and Applied Genetics

, Volume 84, Issue 1, pp 6–9

Characterization of a rice (Oryza sativa L.) mutant deficient in the heme domain of nitrate reductase


  • H. Hasegawa
    • Research Institute for Advanced Science and Technology
  • T. Katagiri
    • Faculty of AgricultureKagawa University
  • S. Ida
    • Research Institute for Food ScienceKyoto University
  • O. Yatou
    • Institute of Radiation Breeding, NIAR
  • M. Ichii
    • Faculty of AgricultureKagawa University

DOI: 10.1007/BF00223974

Cite this article as:
Hasegawa, H., Katagiri, T., Ida, S. et al. Theoret. Appl. Genetics (1992) 84: 6. doi:10.1007/BF00223974


Biochemical and genetical characterization of a rice nitrate reductase (NR)-deficient mutant, M819, which had been isolated as a chlorate-resistant mutant, was carried out. In M819, leaf NADH-NR activity was found to be about 10% of that of the wild-type cv ‘Norin 8’, while NADPH-NR activity was higher than that in the wild-type; FMNH2-NR and MV-NR activities were also 10% of those of the wild type; BPB-NR activity was higher than that of the wild type; and xanthine dehydrogenase activity was revealed to be present in both. These results suggest that the mutant line M819 lacks the functional heme domain of the NADH-NR polypeptide due to a point mutation or a small deletion within the coding region of the structural gene. Chlorate resistance in M819 was transmitted by a single recessive nuclear gene.

Key words

Oryza sativa L.Nitrate reductaseDeficient mutantChlorate resistanceHeme domain



Nitrate reductase


nitrite reductase


reduced flavin nucleotide


reduced methyl viologen


reduced bromphenol blue


xanthine dehydrogenase

Copyright information

© Springer-Verlag 1992