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Detection of genetic diversity in closely related bread wheat using microsatellite markers

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Abstract

Wheat microsatellites (WMS) were used to estimate the extent of genetic diversity among 40 wheat cultivars and lines, including mainly European elite material. The 23 WMS used were located on 15 different chromosomes, and revealed a total of 142 alleles. The number of alleles ranged from 3 to 16, with an average of 6.2 alleles per WMS. The average dinucleotide repeat number ranged from 13 to 41. The correlation coefficient between the number of alleles and the average number of repeats was only slight (r s = 0.55). Based on percentage difference a dendrogram is presented, calculated by the WMS-derived data. All but two of the wheat cultivars and lines could be distinguished. Some of the resulting groups are strongly related to the pedigrees of the appropriate cultivars. Values for co-ancestry (f) of 179 pairs of cultivars related by their pedigrees (f⩾0.1) averaged 0.29. Genetic similarity (GS) based on WMS of the same pairs averaged 0.44. The rank correlation for these pairs was slight, with r s = 0.55, but highly significant (P<0.001). The results suggest that a relatively small number of microsatellites can be used for the estimation of genetic diversity and cultivar identification in elite material of hexaploid bread wheat.

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References

  • Chen HB, Martin JM, Lavin M, Talbert LE (1994) Genetic diversity in hard red spring wheat based on sequence-tagged-site PCR markers. Crop Sci 34:1629–1632

    Google Scholar 

  • Cox TS, Lookhart GL, Walker DE, Harrell LG, Albers LD, Rodgers DM (1985) Genetic relationships among hard red winter wheat cultivars as evaluated by pedigree analysis and gliadin polyacrylamide-gel electrophoretic patterns. Crop Sci 25:1058–1063

    Google Scholar 

  • Devos KM, Bryan GJ, Collins AJ, Stephenson P, Gale MD (1995) Application of two microsatellite sequences in wheat storage proteins as molecular markers. Theor Appl Genet 90:247–252

    CAS  Google Scholar 

  • Dweikat I, MacKenzie S, Levy M, Ohm H (1993) Pedigree assessment using RAPD-DGGE in cereal crop species. Theor Appl Genet 83:497–505

    Google Scholar 

  • Graner A, Ludwig WF, Melchinger AE (1995) Relationships among European barley germplasm. II. Comparison of RFLP and pedigree data. Crop Sci 34:1199–1205

    Google Scholar 

  • He S, Ohm H, Mackenzie S (1992) Detection of DNA sequence polymorphisms among wheat varieties. Theor Appl Genet 84:573–578

    Google Scholar 

  • Levinson G, Gutman GA (1987) Slipped strand mispairing: a major mechanism for DNA sequence evolution. Mol Biol Evol 4:203–221

    CAS  PubMed  Google Scholar 

  • Martynov SP, Dobrotvorskaya TV, Stehno Z, Dotlacil L, Faberova I, Holubec V (1992) Catalogue — genealogies and gene alleles identified in 31000 cultivars and lines of wheat. Research Institute of Crop Production, Prague

    Google Scholar 

  • Melchinger AE, Graner A, Singh M, Messmer MM (1995) Relationships among European barley germplasm I. Genetic diversity among winter and spring cultivars revealed by RFLPs. Crop Sci 34:1191–1199

    Google Scholar 

  • Murphy GL, Connell TD, Barritt DS, Koomey M, Cannon JG (1989) Phase variation of gonococcal protein. II. Regulation of gene expression by slipped-strand mispairing of a repetitive DNA sequence. Cell 56:539–547

    Google Scholar 

  • Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5269–5273

    CAS  PubMed  Google Scholar 

  • Plaschke J, Börner A, Wendehake K, Ganal MW, Röder MS (1995) The use of wheat aneuploids for the chromosomal assignment of microsatellite loci. Euphytica (in press)

  • Podani J (1990) SYN-TAX III-pc-supplement3: Macintosh version. Abstr Bot 14:23–29

    Google Scholar 

  • Röder MS, Plaschke J, König SU, Börner A, Sorrells ME, Tanksley SD, Ganal MW (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet 246:327–333

    PubMed  Google Scholar 

  • Rongwen J, Akkaya MS, Bhagwat AA, Lavi U, Cregan PB (1995) The use of microsatellite DNA markers for soybean genotype identification. Theor Appl Genet 90:43–48

    CAS  Google Scholar 

  • Saghai Maroof MA, Biyashev RM, Yang GP, Zhang Q, Allard RW (1994) Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics. Proc Natl Acad Sci USA 91:5466–5470

    PubMed  Google Scholar 

  • Scarth R (1981) The genetic control of daylength response in wheat. Ph. D thesis, The University of Cambridge

  • Sears ER (1966) Nuilisomic-tetrasomic combinations in hexaploid wheat. In: Riley R, Lewis KR (eds) Chromosome manipulation and plant genetics. Oliver and Boyd, Edinburgh, pp 29–45

    Google Scholar 

  • Sears ER, Sears LMS (1978) The telocentric chromosomes of common wheat. In: Ramanujam S (ed) Proc 5th Int Wheat Genet Symp. Indian Soc Genet Plant Breed, New Delhi, pp 389–407

    Google Scholar 

  • Sheen SJ, Snyder LA (1964) Studies on the inheritance of resistance to six stem rust cultures using chromosome substitution lines of a Marquis wheat selection. Can J Genet Cytol 6:74–82

    Google Scholar 

  • Talbert LE, Blake NK, Chee PW, Blake TK, Magyar GM (1994) Evaluation of “sequence-tagged-site” PCR products as molecular markers in wheat. Theor Appl Genet 87:789–794

    CAS  Google Scholar 

  • Tinker NA, Fortin MG, Mather DE (1993) Random amplified polymorphic DNA and pedigree relationships in spring barley. Theor Appl Genet 85:976–984

    Google Scholar 

  • Tautz D, Schlötterer C (1994) Simple sequences. Curr Opin Genet Dev 4:832–837

    Google Scholar 

  • Vaccino P, Accerbi M, Corbellini M (1993) Cultivar identification in T. aestivum using highly polymorphic RFLP probes. Theor Appl Genet 86:833–836

    Google Scholar 

  • Viglasi P (1968) Short-strawed mutants of Karcag 522 winter wheat induced by gamma rays. Acta Agron 17:205–214

    Google Scholar 

  • Weber JL (1990) Informativeness of human (dC-dA)n·(dG-dT) n polymorphisms. Genomics 7:524–530

    CAS  PubMed  Google Scholar 

  • Wolff RK, Plaetke R, Jeffreys AJ, White R (1989) Unequal crossingover between homologous chromosomes is not the major mechanism involved in the generation of new alleles at VNTR loci. Genomics 5:382–384

    Google Scholar 

  • Ynag GP, Saghai Maroof MA, Xu CG, Qifa Zhang, Biyashev RM (1994) Comparative analysis of microsatellite DNA polymorphism in landraces and cultivars of rice. Mol Gen Genet 245:187–194

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstraße 3, 06466, Gatersleben, Germany

    J. Plaschke, M. W. Ganal & M. S. Röder

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  1. J. Plaschke
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  2. M. W. Ganal
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  3. M. S. Röder
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Communicated by G. Wenzel

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Plaschke, J., Ganal, M.W. & Röder, M.S. Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theoret. Appl. Genetics 91, 1001–1007 (1995). https://doi.org/10.1007/BF00223912

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  • DOI: https://doi.org/10.1007/BF00223912

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