Theoretical and Applied Genetics

, Volume 88, Issue 3, pp 273–278

An RFLP linkage map for loblolly pine based on a three-generation outbred pedigree

  • M. E. Devey
  • T. A. Fiddler
  • B. H. Liu
  • S. J. Knapp
  • D. B. Neale
Article

DOI: 10.1007/BF00223631

Cite this article as:
Devey, M.E., Fiddler, T.A., Liu, B.H. et al. Theoret. Appl. Genetics (1994) 88: 273. doi:10.1007/BF00223631

Abstract

A genetic linkage map for loblolly pine (Pinus taeda L.) was constructed using segregation data from a three-generation outbred pedigree consisting of four grandparents, two parents, and 95 F2 progeny. The map was based predominantly on restriction fragment length polymorphism (RFLP) loci detected by cDNA probes. Sixty-five cDNA and three genomic DNA probes revealed 90 RFLP loci. Six polymorphic isozyme loci were also scored. One-fourth (24%) of the cDNA probes detected more than 1 segregating locus, an indication that multigene families are common in pines. As many as six alleles were observed at a single segregating locus among grandparents and it was not unusual for the progeny to segregate for three or four alleles per locus. Multipoint linkage analysis placed 73 RFLP and 2 isozyme loci into 20 linkage groups; the remaining 17 RFLP and 4 isozyme loci were unlinked. The mapped RFLP probes provide a new set of codominant markers for genetic analyses in loblolly pine.

Key words

RFLPGenetic linkage mappingPinus taeda L.

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • M. E. Devey
    • 1
  • T. A. Fiddler
    • 2
  • B. H. Liu
    • 3
  • S. J. Knapp
    • 4
  • D. B. Neale
    • 5
  1. 1.Division of Forestry/CSIROCanberraAustralia
  2. 2.School of MedicineOregon Health SciencesPortlandUSA
  3. 3.Department of ForestryNorth Carolina State UniversityRaleighUSA
  4. 4.Department of Crop and Soil SciencesOregon State UniversityCorvallisUSA
  5. 5.Pacific Southwest Research StationInstitute of Forest Genetics, USDA/Forest ServiceBerkeleyUSA