Immunogenetics

, Volume 37, Issue 1, pp 1–11

Mhc-DRB diversity of the chimpanzee (Pan troglodytes)

  • M. Kenter
  • N. Otting
  • J. Anholts
  • M. Jonker
  • R. Schipper
  • R. E. Bontrop
Original articles

DOI: 10.1007/BF00223539

Cite this article as:
Kenter, M., Otting, N., Anholts, J. et al. Immunogenetics (1992) 37: 1. doi:10.1007/BF00223539

Abstract

Fifty-four chimpanzee Patr-DRB and five human HLA-DRB second exons were cloned and sequenced from thirty-five chimpanzees and four B-cell lines and compared with known Mhc-DRB sequences of these two species. Equivalents of the HLA-DRB1*02,-DRB1*03, -DRB1*07 allelic lineages and the HLA-DRB3,-DRB4, -DRB5, -DRB6, and -DRB7 loci were all found in the chimpanzee. In addition, two chimpanzee Patr-DRB lineages (Patr-DRBX and -DRBY) were found for which no human counterparts have been described. None of the Patr-DRB sequences is identical to known HLA-DRB sequences. The Patr-DRB1*0702 and HLA-DRB1*0701 alleles are the most similar sequences in a comparison between the two species and differ by only two nucleotides out of 246 sequences. Equivalents of the HLA-DRB1*01,-DRB1*04, and -DRB1*09 alleles were not found in our sample of chimpanzees. A per locus comparison of the number of Patr-DRB alleles with the HLA-DRB alleles shows that the Patr-DRB3, -DRB4, -DRB5, and -DRB6 locus are, thus far, more polymorphic than ther human homologs. The polymorphism of the Patr-DRB1 locus seems to be less extensive than that reported for the HLA-DRB1 locus. Nevertheless, the Patr-DRB1 locus seems to be the most polymorphic of the Patr-DRB loci. Phylogenetic analyses indicate that the HLA-DRB1*09 allele may have originated from a recombination between a Mhc-DRB5 allele and the DRB1 allele of a Mhc-DR7 haplotype. Although recombination seems to increase the diversity of the Patr-DRB alleles, its contribution to the generation of Patr-DRB variation is probably low. Hence, most Patr-DRB diversity presumably accumulated via recurrent point mutations. Finally, two distinct PAtr-DRB haplotypes are deduced, one of which (the chimpanzee equivalent of the HLA-Dr7 haplotype) is probably older than 6–8 million years.

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • M. Kenter
    • 1
    • 2
  • N. Otting
    • 1
  • J. Anholts
    • 2
  • M. Jonker
    • 1
  • R. Schipper
    • 2
  • R. E. Bontrop
    • 1
  1. 1.ITRI-TNOHV RijswijkThe Netherlands
  2. 2.Department of Immunohematology and Blood BankLeiden University HospitalThe Netherlands