Theoretical and Applied Genetics

, Volume 93, Issue 8, pp 1202–1210

PCR-based fingerprinting using AFLPs as a tool for studying genetic relationships in Lactuca spp.


  • M. Hill
    • Department of BiologyUniversity of Massachusetts
  • H. Witsenboer
    • Department of Vegetable CropsUniversity of California
  • M. Zabeau
    • Keygene BV
  • P. Vos
    • Keygene BV
  • R. Kesseli
    • Department of BiologyUniversity of Massachusetts
  • R. Michelmore
    • Department of Vegetable CropsUniversity of California

DOI: 10.1007/BF00223451

Cite this article as:
Hill, M., Witsenboer, H., Zabeau, M. et al. Theoret. Appl. Genetics (1996) 93: 1202. doi:10.1007/BF00223451


AFLP markers were evaluated for determining the phylogenetic relationships Lactuca spp. Genetic distances based on AFLP data were estimated for 44 morphologically diverse lines of cultivated L. sativa and 13 accessions of the wild species L. serriola, L. saligna, L. virosa, L. perennis, and L. indica. The same genotypes were analyzed as in a previous study that had utilized RFLP markers. The phenetic tree based on AFLP data was consistent with known taxonomic relationships and similar to a tree developed with RFLP data. The genetic distance matrices derived from AFLP and RFLP data were compared using least squares regression analysis and, for the cultivar data, by principal component analysis. There was also a positive linear relationship between distance estimates based on AFLP data and kinship coefficients calculated from pedigree data. AFLPs represent reliable PCR-based markers for studies of genetic relationships at a variety of taxonomic levels.

Key words

AFLP markerLactucaLettucePhylogenetic analysis

Copyright information

© Springer-Verlag 1996