Theoretical and Applied Genetics

, Volume 86, Issue 6, pp 705–712

A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome

Authors

  • A. Kleinhofs
    • Department of Crop and Soil SciencesWashington State University
    • Department of Genetics and Cell BiologyWashington State University
  • A. Kilian
    • Department of Crop and Soil SciencesWashington State University
  • M. A. Saghai Maroof
    • Department of Crop and Soil Environmental SciencesVirginia Polytechnic Institute and State University
  • R. M. Biyashev
    • Department of Crop and Soil Environmental SciencesVirginia Polytechnic Institute and State University
  • P. Hayes
    • Crop Sciences DepartmentOregon State University
  • F. Q. Chen
    • Crop Sciences DepartmentOregon State University
  • N. Lapitan
    • Department of AgronomyColorado State University
  • A. Fenwick
    • Department of AgronomyColorado State University
  • T. K. Blake
    • Department of Plant and Soil SciencesMontana State University
  • V. Kanazin
    • Department of Plant and Soil SciencesMontana State University
  • E. Ananiev
    • Department of Plant and Soil SciencesMontana State University
  • L. Dahleen
    • USDA-ARS, Northern Crop Science Laboratory
  • D. Kudrna
    • Department of Crop and Soil SciencesWashington State University
  • J. Bollinger
    • Department of Crop and Soil SciencesWashington State University
  • S. J. Knapp
    • Crop Sciences DepartmentOregon State University
  • B. Liu
    • Crop Sciences DepartmentOregon State University
  • M. Sorrells
    • Department of Plant BreedingCornell University
  • M. Heun
    • Department of Plant BreedingCornell University
  • J. D. Franckowiak
    • Department of Crop and Weed SciencesNorth Dakota State University
  • D. Hoffman
    • USDA-ARS, National Small Grains Germplasm Research Facility
  • R. Skadsen
    • Cereal Crops Research Unit
  • B. J. Steffenson
    • Department of Plant PathologyNorth Dakota State University
Article

DOI: 10.1007/BF00222660

Cite this article as:
Kleinhofs, A., Kilian, A., Saghai Maroof, M.A. et al. Theoret. Appl. Genetics (1993) 86: 705. doi:10.1007/BF00222660

Abstract

A map of the barley genome consisting of 295 loci was constructed. These loci include 152 cDNA restriction fragment length polymorphism (RFLP), 114 genomic DNA RFLP, 14 random amplified polymorphic DNA (RAPD), five isozyme, two morphological, one disease resistance and seven specific amplicon polymorphism (SAP) markers. The RFLP-identified loci include 63 that were detected using cloned known function genes as probes. The map covers 1,250 centiMorgans (cM) with a 4.2 cM average distance between markers. The genetic lengths of the chromosomes range from 124 to 223 cM and are in approximate agreement with their physical lengths. The centromeres were localized to within a few markers on all of the barley chromosomes except chromosome 5. Telomeric regions were mapped for the short (plus) arms of chromosomes 1, 2 and 3 and the long (minus) arm of chromosomes 7.

Key words

RFLPMappingBarleyGenomeCentromeres

Copyright information

© Springer-Verlag 1993