Theoretical and Applied Genetics

, Volume 86, Issue 6, pp 689–693

RFLP mapping of the ym4 virus resistance gene in barley

  • A. Graner
  • E. Bauer

DOI: 10.1007/BF00222657

Cite this article as:
Graner, A. & Bauer, E. Theoret. Appl. Genetics (1993) 86: 689. doi:10.1007/BF00222657


RFLP (restriction fragment length polymorphism) mapping of a recessive gene (ym4) conferring resistance to barley yellow mosaic and barley mild mosaic virus was performed using progeny of 86 F1 anther-derived doubled haploid lines. Two closely linked RFLP markers that flank the gene at a distance of 1.2 centiMorgans were identified. Using one of these markers (MWG10) we obtained a clear differentiation between resistant and susceptible German cultivars. An analysis of a series of unrelated barley lines with probe MWG10 did not reveal additional RFLP fragments. The use of this probe for both marker-assisted selection and the generation of a high-density map around the resistance locus is discussed.

Key words

Restriction fragment length polymorphismLinkage mapBaYMVBaMMV

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • A. Graner
    • 1
  • E. Bauer
    • 1
  1. 1.Institute for Resistance GeneticFederal Biological Research Centre for Agriculture and ForestryGrünbachGermany