Cell and Tissue Research

, Volume 257, Issue 1, pp 179–185

Localisation of islet amyloid peptide in lipofuscin bodies and secretory granules of human B-cells and in islets of type-2 diabetic subjects


  • Anne Clark
    • Diabetes Research Laboratories, Radcliffe Infirmary
    • Department of Human Anatomy
  • Catherine A. Edwards
    • Diabetes Research Laboratories, Radcliffe Infirmary
  • Lynne R. Ostle
    • Department of Human Anatomy
  • Robert Sutton
    • Nuffield Department of SurgeryJohn Radcliffe Hospital
  • Jonathan B. Rothbard
    • Imperial Cancer Research Fund
  • John F. Morris
    • Department of Human Anatomy
  • Robert C. Turner
    • Diabetes Research Laboratories, Radcliffe Infirmary

DOI: 10.1007/BF00221649

Cite this article as:
Clark, A., Edwards, C.A., Ostle, L.R. et al. Cell Tissue Res. (1989) 257: 179. doi:10.1007/BF00221649


Islet amyloid peptide (or diabetes-associated peptide), the major component of pancreatic islet amyloid found in type-2 diabetes, has been identified by electronmicroscopic immunocytochemistry in pancreatic B-cells from five non-diabetic human subjects, and in islets from five type-2 diabetic patients. The greatest density of immunoreactivity for islet amyloid peptide was found in electrondense regions of some lysosomal or lipofuscin bodies. The peptide was also localised by quantification of immunogold in the secretory granules of B-cells, and was present in cytoplasmic lamellar bodies. Acid phosphatase activity was also demonstrated in these organelles. Immunoreactivity for insulin was found in some lysosomes. These results suggest that islet amyloid peptide is a constituent of normal pancreatic B-cells, and accumulates in lipofuscin bodies where it is presumably partially degraded. In islets from type-2 diabetic subjects, amyloid fibrils and lipofuscin bodies in B-cells showed immunoreactivity for the amyloid peptide. Abnormal processing of the peptide within B-cells could lead to the formation of islet amyloid in type-2 diabetes.

Key words

Islet amyloid peptidePancreatic isletsType-2 diabetesInsulinLysosomesSecretory granulesMan

Copyright information

© Springer-Verlag 1989