Cell and Tissue Research

, Volume 257, Issue 1, pp 149–153

Calbindin immunoreactivity in the neurons of the spinal cord and dorsal root ganglion of the domestic fowl

  • C. A. Lunam

DOI: 10.1007/BF00221645

Cite this article as:
Lunam, C.A. Cell Tissue Res. (1989) 257: 149. doi:10.1007/BF00221645


The distribution of the vitamin D-dependent calcium-binding protein, calbindin, was mapped in the brachial spinal cord and in the 15th dorsal root ganglion of the domestic fowl, using fluorescence immunohistochemistry. Cell somata of the dorsal root ganglion ranged in area from 200 μm2 to 2000 μm2. Sixteen percent of cell bodies displayed calbindin immunoreactivity. Reactivity occurred in both the small and large sensory neurons. These were randomly distributed within each ganglion. In the spinal cord, calbindin immunoreactivity was intense in Lissauer's tract, and in nerve fibres and nerve cell bodies within laminae 1 to 3 of the dorsal horn. Scattered varicose fibres were observed in laminae 4 to 7. Immunoreactivity was intense in laminae 10 where nerve fibres formed a meshwork around the central canal. Immunoreactive perikarya were occasionally observed in the outer region of lamina 10 and between laminae 8 and 9. The perikarya of the large motoneurons of lamina 9 were not reactive although they were enmeshed in calbindin-immunoreactive fibres.

Key words

CalbindinBrachial spinal cordDorsal root ganglionImmunohistochemistryDomestic fowl

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • C. A. Lunam
    • 1
  1. 1.Department of Anatomy and HistologyCentre for Neuroscience, Flinders University School of MedicineAustralia
  2. 2.Department of Anatomy and HistologySchool of Medicine, Flinders University of South AustraliaBedford ParkAustralia