Theoretical and Applied Genetics

, Volume 90, Issue 1, pp 11–16

Nuclear DNA content of Vitis species, cultivars, and other genera of the Vitaceae

  • M. A. Lodhi
  • B. I. Reisch
Article

DOI: 10.1007/BF00220990

Cite this article as:
Lodhi, M.A. & Reisch, B.I. Theoret. Appl. Genetics (1995) 90: 11. doi:10.1007/BF00220990

Abstract

The nuclear DNA content was analyzed in Vitis species, hybrid cultivars, and genera of the Vitaceae using flow cytometry. Significant variation was found among Vitis species, hybrids, and other genera of the Vitaceae (Ampelopsis and Parthenocissus). DNA content was estimated to range from 0.98 to 1.05 pg/2C within V. labrusca (ns) and 0.86 to 1.00 pg/2C within V. vinifera (ns). Genotypes from Vitis and Parthenocissus were similar in nuclear DNA content (approximately 1.00 pg/2C) whereas they differed significantly from Ampelopsis (1.39 pg/2C). No correlation between DNA content and the center of origin of genotypes of the Vitaceae was noted. Based on the present study, the Vitis genome size is 475 Mbp, 96% of which is non-coding. Knowledge of DNA content is useful in order to understand the complexity of the Vitis genome and to establish a relationship between the genetic and physical map for map-based cloning.

Key words

AmpelopsisFlow cytometryGrapevinesParthenocissus

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • M. A. Lodhi
    • 1
  • B. I. Reisch
    • 1
  1. 1.Departmet of Horticultural Sciences, New York State Agricultural Experiment StationCornell UniversityGenevaUSA
  2. 2.Cold Spring Harbor LaboratoryCold Spring HarborUSA