Theoretical and Applied Genetics

, Volume 91, Issue 2, pp 242–247

Inheritance and mechanism of resistance to herbicides inhibiting acetolactate synthase in Sonchus oleraceus L.

  • P. Boutsalis
  • S. B. Powles
Article

DOI: 10.1007/BF00220884

Cite this article as:
Boutsalis, P. & Powles, S.B. Theoret. Appl. Genetics (1995) 91: 242. doi:10.1007/BF00220884

Abstract

A biotype of Sonchus oleraceus L. (Compositae) has developed resistance to herbicides inhibiting acetolactate synthase (ALS) following field selection with chlorsulfuron for 8 consecutive years. The aim of this study was to determine the inheritance and mechanism of resistance in this biotype. Determination of ALS activity and inhibition kinetics revealed that Km and Vmax did not vary greatly between the resistant and susceptible biotypes. ALS extracted from the resistant biotype was resistant to five ALS-inhibiting herbicides in an in vitro assay. ALS activity from the resistant biotype was 14 19, 2, 3 and 3 times more resistant to inhibition by chlorsulfuron, sulfometuron, imazethapyr, imazapyr and flumetsulam, respectively, than the susceptible biotype. Hybrids between the resistant and a susceptible biotype were produced, and inheritance was followed through the F1, F2 and F3 generations. F1 hybrids displayed a uniform intermediate level of resistance between resistant and susceptible parents. Three distinct phenotypes, resistant, intermediate and susceptible, were identified in the F2 generation following chlorsulfuron application. A segregation ratio of 1∶2∶1 was observed, indicative of the action of a single, nuclear, incompletely dominant gene. F3 families, derived from intermediate F2 individuals, segregated in a similar manner. Resistance to herbicides inhibiting ALS in this biotype of S. oleraceus is due to the effect of a single gene coding for a resistant form of the target enzyme, ALS.

Key words

Sonchus oleraceus L. Acetolactate synthase Inheritance Herbicide resistance 

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • P. Boutsalis
    • 1
  • S. B. Powles
    • 1
  1. 1.Department of Crop ProtectionWaite Agricultural Research Institute, The University of AdelaideGlen OsmondAustralia