Cell and Tissue Research

, Volume 167, Issue 1, pp 117–123

Fate of 3H-thymidine labelled myogenic cells in regeneration of muscle isografts

  • E. Gutmann
  • V. Mareš
  • J. Štichová
Article

DOI: 10.1007/BF00220163

Cite this article as:
Gutmann, E., Mareš, V. & Štichová, J. Cell Tissue Res. (1976) 167: 117. doi:10.1007/BF00220163

Summary

Intact and denervated extensor digitorum longus (EDL) muscles of 20-day-old inbred Lewis-Wistar rats were labelled with 3H-thymidine. Ninety minutes after the injection of the isotope 4.0% of the nuclei were labelled in the intact (i.e. innervated) and 9.6% in the muscles, denervated 3 days before administration of the isotope. The labelled EDL muscles were grafted into the bed of the previously removed EDL muscles of inbred animals and these isografts were studied 30 days later.

In the EDL muscles, regenerated from innervated isografts only occasionally labelled endothelial cells were found whereas in the muscles regenerated from denervated isografts also parenchymal muscle nuclei were regularly labelled. The incidence of labelled nuclei in the regenerated EDL muscles was, however, about 20 times lower than in the donor EDL muscles. The present experiments provide a direct proof of utilization of donor satellite cell nuclei for regeneration in grafted muscle tissue. With respect to the low incidence of labelled nuclei in regenerated EDL muscles, other sources of cells apparently also contribute to the regeneration process.

Key words

Skeletal muscle Transplantation Muscle regeneration Historadiography 

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • E. Gutmann
    • 1
  • V. Mareš
    • 1
  • J. Štichová
    • 1
  1. 1.Institute of PhysiologyCzechoslovak Academy of SciencesPragueCzechoslovakia
  2. 2.Institute of PhysiologyCzechoslovak Academy of SciencesPragueCSSR

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