Theoretical and Applied Genetics

, Volume 81, Issue 4, pp 437–444

Stable transformation of barley via PEG-induced direct DNA uptake into protoplasts

  • P. A. Lazzeri
  • R. Brettschneider
  • R. Lührs
  • H. Lörz
Article

DOI: 10.1007/BF00219433

Cite this article as:
Lazzeri, P.A., Brettschneider, R., Lührs, R. et al. Theoret. Appl. Genetics (1991) 81: 437. doi:10.1007/BF00219433

Summary

Protoplasts isolated from a barley cell suspension (cv Dissa) were transformed with plasmid DNA containing the neomycinphosphotransferase II (NPT) and β-glucuronidase (GUS) genes, using polyethyleneglycol (PEG) to induce DNA uptake. Transformed microcalli were selected in media containing G418 sulphate. NPT activity was detected in all antibiotic-resistant cell lines, but not all NPT-positive cell lines had GUS activity. Southern analysis confirmed the presence of sequences homologous to the APT and GUS genes in DNA of G418-resistant callus.

Key words

Hordeum vulgare Protoplasts DNA uptake Stable transformation Cereals 
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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • P. A. Lazzeri
    • 1
  • R. Brettschneider
    • 1
  • R. Lührs
    • 1
  • H. Lörz
    • 1
  1. 1.Institute for General Botany (AMP II), University of HamburgHamburgFRG

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