Cell and Tissue Research

, Volume 170, Issue 3, pp 289–303

Mast cell activation and tissue cell proliferation

  • Klas Norrby
  • Lennart Enerbäck
  • Lennart Franzén
Article

DOI: 10.1007/BF00219412

Cite this article as:
Norrby, K., Enerbäck, L. & Franzén, L. Cell Tissue Res. (1976) 170: 289. doi:10.1007/BF00219412

Summary

The effect of mast cell activation and degranulation on the proliferation in the intact mesentery was studied in Sprague-Dawley rats. Mast cell activation was achieved by a single intraperitoneal injection of Compound 48/80.

The proliferation was studied using three independent methods for estimation of cell production and DNA synthesis: 1. the mitotic index, 2. the relative number of cells having a DNA content in the S and G2 regions, by Feulgen photometric measurement in individual cells, and 3. the specific DNA activity, employing a method which combines a liquid scintillation technique after an intravenous injection of 3H-thymidine and Feulgen photometric determination of the DNA content per membrane preparation.

It was found that the proliferation of the normal mesenchymal cells adjacent to the activated and degranulated mast cells in the mesentery was significantly increased within 24 and 32 h, the maximum increase being more than 20-fold compared to untreated controls. The results suggest that the common type of mast cell may have a pathophysiological function related to stimulation of local cell proliferation.

Key words

Mast cell Compound 48/80 Cell proliferation Mesentery DNA Mitosis 

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Klas Norrby
    • 1
  • Lennart Enerbäck
    • 1
  • Lennart Franzén
    • 1
  1. 1.Department of Pathology IIUniversity of LinköpingSweden
  2. 2.Department of Pathology IIUniversity of LinköpingLinköpingSweden

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