Molecular and Cellular Biochemistry

, Volume 92, Issue 2, pp 129–135

Stimulation and inhibition of cardiac myocyte proliferation in vitro

  • Elissavet Kardami
Original Article

DOI: 10.1007/BF00218130

Cite this article as:
Kardami, E. Mol Cell Biochem (1990) 92: 129. doi:10.1007/BF00218130

Summary

We have examined the effect of crude cardiac tissue extracts as well as that of several growth factors and triiodothyronin (T3) on DNA synthesis of cardiac myocytes in culture. Extracts from embryonic and adult cardiac tissue stimulated DNA synthesis of myocytes. Atrial myocytes exhibited overall higher degree of stimulation than their ventricular counterparts and extracts from adult atrial tissue had the highest apparent mitogenic activity for atrial myocytes. We have shown that adult heart contains basic fibroblast growth factor (bFGF), especially in the atria [1]. Transforming growth factor β (TGFβ) and insulin-like growth factors (IGFs) are also accumulated in cardiac tissues [2, 3]. We found that bFGF and the IGFs stimulate myocyte cell proliferation and DNA synthesis. These factors also stimulate cardiac non-muscle proliferation, especially in the presence of serum. TGFβ inhibited proliferation and DNA synthesis and cancelled the effect of bFGF or IGFs on the myocytes. T3 also diminished the bFGF-induced mitogenic stimulation of cardiomyocytes. Our data suggest that these factors may be involved in the regulation of cardiomyocyte proliferation in vivo.

Key words

cardiocytescell proliferationgrowth factorsDNA synthesis

Abbreviations

bFGF

basic Fibroblast Growth Factor

BSA

Bovine Serum Albumin

DM

Defined Medium

Fes

Fetal calf serum

FITC

Fluorescein

IGF

Insulin-like Growth Factor

IgG

Immunoglobulin

LI

Labeling Index

PBS

Phosphate Buffered Saline

T3

Triiodothyronine

TGFβ

Transforming Growth Factor β

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Elissavet Kardami
    • 1
  1. 1.St. Boniface General Hospital Research Centre, Division of Cardiovascular SciencesUniversity of ManitobaWinnipegCanada