Human Genetics

, Volume 90, Issue 5, pp 505–510

Cloning and chromosome mapping of human retinoid X receptor β: selective amino acid sequence conservation of a nuclear hormone receptor in mammals

  • K. Fleischhauer
  • O. W. McBride
  • J. P. DiSanto
  • K. Ozato
  • S. Y. Yang
Original Investigations

DOI: 10.1007/BF00217449

Cite this article as:
Fleischhauer, K., McBride, O.W., DiSanto, J.P. et al. Hum Genet (1993) 90: 505. doi:10.1007/BF00217449

Abstract

The murine retinoid X receptor β (mRXRβ) is a nuclear hormone receptor that activates transcription of murine major histocompatibility complex (MHC) class I genes in response to retinoic acid. In this study, the human RXRβ gene was mapped onto the short arm or centromeric region of chromosome 6 (6pter-q13), which also harbors the MHC. Chromosomal localization was performed by Southern hybridization of genomic DNA from human rodent cell hybrids with the mRXRβ gene as a probe. In addition, a full-length cDNA clone encoding a human RXRβ was isolated by nucleic acid screening of a human cDNA library with a fragment of the mRXRβ gene as a probe. Comparison of the nucleotide-coding sequences of the human and the murine RXRβ revealed a predominance of third base substitutions, resulting in selective conservation of the predicted amino acid sequence of the proteins. The overall sequence homology was 97.6% on the amino acid level as opposed to 91.6% on the nucleotide level. In Northern hybridization experiments with the human cDNA as a probe, RXRβ gene transcripts were detected in a variety of human tumor cell lines, regardless of whether these cell lines expressed MHC class I genes.

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • K. Fleischhauer
    • 1
  • O. W. McBride
    • 2
  • J. P. DiSanto
    • 3
  • K. Ozato
    • 4
  • S. Y. Yang
    • 1
  1. 1.Laboratory of Biochemical Immunogenetics, Memorial Sloan Kettering Cancer CenterNew YorkUSA
  2. 2.Laboratory of Biochemistry, Division of Cancer Biology and DiagnosisNational Cancer InstituteBethesdaUSA
  3. 3.Laboratory of Cellular Immunobiology, Memorial Sloan Kettering Cancer CenterNew YorkUSA
  4. 4.Laboratory of Development and Molecular Immunity, Nationl Institute of Child Health and Human DevelopmentBethesdaUSA
  5. 5.Department of HematologyOspedale San RaffaeleMilanoItaly

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