Summary
In situ hybridization, under low stringency conditions with two alphoid DNA probes (pYα1 and p82H) labeled with digoxigenin-dUTP, decorated all the centromeres of the human karyotype. However, signals were also detected on the long arm of chromosome 2 at approximately q21.3–q22.1. Since it is supposed that human chromosome 2 originated by the telomeric fusion of two ancestral primate chromosomes, these findings indicate that not only the telomeric sequences, but also the ancestral centromere (or at least its alphoid sequences), have been conserved.
References
Allshire RC, Gosden JR, Cross SH, Cranston G, Rout D, Sugawara N, Szostak JW, Fantes PA, Hastig ND (1988) Telomeric repeat from T. thermophila cross hybridizes with human telomers. Nature 332:656–659
Dutrillaux B (1975) Sur la nature et al origine des chromosomes humains. L' Expansion Scientifique, Paris
Hecht F, Ramesh KH, Lockwood DH (1990) A guide to fragile sites on human chromosomes. Cancer Genet Cytogenet 44:37–46
Ijdo JW, Baldini A, Wells RAW, Reeders ST (1991) An ancestral telomere-telomere fusion maps close to the fragile site at 2q13 (abstract). In: Genome mapping and sequencing. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Lejeune J, Dutrillaux B, Rethore MO, Prieur M (1973) Comparison de la structure fine des chromatides d'Homo sapiens et de Pan troglodytes. Chromosoma 43:423–444
Manuelidis L, Wu JC (1978) Homology between human and simian repeated DNA. Nature 276:92–94
Maraschio P, Zuffardi O, Caiulo A, Dainotti E, Piantanida M, Rivera H, Tupler R (1990) Deletion of specific sequences or modification of centromeric chromatin are responsible for Y chromosome centromere inactivation. Hum Genet 85:491–494
Tyler-Smith C, Brown WRA (1987) Structure of the major block of alphoid satellite DNA on the human Y chromosome. J Mol Biol 195:457–470
Waye JS, Mitchell AR, Willard HF (1988) Organization and genomic distribution of “82H” alpha satellite DNA. Evidence for a low copy or single-copy alphoid domain located on human chromosome 14. Hum Genet 78:27–32
Willard HF, Waye JS (1987) Hierarchical order in chromosome-specific human alpha satellite DNA. Trends Genet 3:192–198
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Avarello, R., Pedicini, A., Caiulo, A. et al. Evidence for an ancestral alphoid domain on the long arm of human chromosome 2. Hum Genet 89, 247–249 (1992). https://doi.org/10.1007/BF00217134
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00217134