Planta

, Volume 188, Issue 2, pp 164–172

Expression and localization of polygalacturonase during the outgrowth of lateral roots in Allium porrum L.

  • R. Peretto
  • F. Favaron
  • V. Bettini
  • G. De Lorenzo
  • S. Marini
  • P. Alghisi
  • F. Cervone
  • P. Bonfante
Article

DOI: 10.1007/BF00216810

Cite this article as:
Peretto, R., Favaron, F., Bettini, V. et al. Planta (1992) 188: 164. doi:10.1007/BF00216810

Abstract

The presence of polygalacturonase and its correlation with the formation of lateral roots in leek (Allium porrum L.) seedlings have been investigated. During root growth, a steady increase in polygalacturonase activity was associated with that of the lateral root primordia. Fractionation of root extract by fast protein liquid chromatography resolved at least two polygalacturonase isoforms. One of the isoforms, a 75-kdalton protein, strongly reacted on Western blots probed with a polyclonal antibody raised against tomato polygalacturonase. It also reacted with both polyclonal and monoclonal antisera raised against Fusarium moniliforme polygalacturonase. In situ localization with these three antibodies showed that polygalacturonase was present over the meristems of lateral root primordia. Antibodies against pectins (Knox et al. 1990, Planta 181, 512–521) detected large amounts of pectic material filling the area between the apex of the primordium and the mother root tissues. We suggest that a polygalacturonase plays an important role in leek root morphogenesis, particularly during lateral root outgrowth.

Key words

Allium (polygalacturonase)Morphogenesis (lateral root)PectinPolygalacturonase (immunolocalization)Root morphogenesis

Abbreviations

FPLC

fast protein liquid chromatography

RGU

one unit of polygalacturonase activity

SDS-PAGE

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • R. Peretto
    • 1
  • F. Favaron
    • 2
  • V. Bettini
    • 1
  • G. De Lorenzo
    • 3
  • S. Marini
    • 4
  • P. Alghisi
    • 2
  • F. Cervone
    • 3
  • P. Bonfante
    • 1
  1. 1.Dipartimento di Biologia Vegetale dell'UniversitàTorinoItaly
  2. 2.Istituto di Patologia Vegetale, Università di PadovaPadovaItaly
  3. 3.Dipartimento di Biologia VegetaleUniversità di RomaRomaItaly
  4. 4.Dipartimento di Medicina Sperimentale e Scienze Biochimiche dell'Università di RomaRomaItaly