Archives of Environmental Contamination and Toxicology

, Volume 31, Issue 3, pp 404–409

Effects of 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) injected into the yolks of chicken (Gallus domesticus) eggs prior to incubation

Authors

  • D. C. Powell
    • Department of Animal ScienceMichigan State University
  • R. J. Aulerich
    • Department of Animal ScienceMichigan State University
    • Institute for Environmental ToxicologyMichigan State University
  • J. C. Meadows
    • Midwest Science CenterNational Biological Service
  • D. E. Tillitt
    • Midwest Science CenterNational Biological Service
  • J. P. Giesy
    • Institute for Environmental ToxicologyMichigan State University
    • Department of Fisheries and WildlifeMichigan State University
    • Pesticide Research CenterMichigan State University
  • K. L. Stromborg
    • U.S. Fish and Wildlife Service
  • S. J. Bursian
    • Department of Animal ScienceMichigan State University
    • Institute for Environmental ToxicologyMichigan State University
Article

DOI: 10.1007/BF00212680

Cite this article as:
Powell, D.C., Aulerich, R.J., Meadows, J.C. et al. Arch. Environ. Contam. Toxicol. (1996) 31: 404. doi:10.1007/BF00212680

Abstract

The yolks of White Leghorn chicken (Gallus domesticus) eggs were injected prior to incubation with either 3,3′,4,4′,5- pentachlorobiphenyl (PCB 126) at doses ranging from 0.1 to 12.8 μg/kg egg or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) at doses ranging from 0.04 to 0.64 μg/kg egg. Chicks were subjected to necropsy within 24 h of hatching. The brain, bursa, heart, liver, and spleen were removed and weighed. Assessment of the rate of hatching indicated an LD50±S.E. of 2.3±0.19 μg/kg egg (7.1±0.58 nmol/kg egg) for PCB 126 and 0.15±0.012 μg/kg egg (0.47±0.037 nmol/kg egg) for TCDD. No significant differences in the incidence of developmental abnormalities (structural defects and edema) were observed in TCDD-exposed embryos, while PCB 126 caused significantly more developmental abnormalities at 3.2, 6.4, and 12.8 μg/kg egg than the vehicle control. PCB 126 caused lower hatchling weights and greater relative brain, heart, and liver weights when compared to the vehicle control group at a dose of 3.2 μg/kg egg which is greater than the LD50. TCDD at 0.08 μg/kg egg caused relative bursa weights to be less than those of the vehicle control. A toxic equivalency factor (TEF) of 0.07 was determined for PCB 126 in relation to TCDD based on overt lethality.

Copyright information

© Springer-Verlag New York Inc 1996