Cell and Tissue Research

, Volume 244, Issue 3, pp 635–643

Degeneration-regeneration as a mechanism contributing to the fast to slow conversion of chronically stimulated fast-twitch rabbit muscle

  • Alfred Maier
  • Brigitte Gambke
  • Dirk Pette
Article

DOI: 10.1007/BF00212544

Cite this article as:
Maier, A., Gambke, B. & Pette, D. Cell Tissue Res. (1986) 244: 635. doi:10.1007/BF00212544

Summary

Extensor digitorum longus muscles of male adult White New Zealand rabbits were indirectly stimulated at 10 Hz for 12 h daily for periods ranging up to 28 days. After four weeks the stimulated muscles showed a nearly uniform profile of high succinate dehydrogenase activity and, when incubated after acid preincubation for myofibrillar adenosine triphosphatase, displayed more dark- and intermediate-staining fibers than their contralateral counterparts. Muscles stimulated from between 6 to 21 days revealed degenerative foci and phagocytosis of degenerated fibers. These fibers were mostly of the fast-twitch, glycolytic type. Small myofibers, which often contained central nuclei, and structures identified as myoblasts or myotubes, reacted with a monoclonal antibody prepared against embryonic myosin heavy chains. The data suggest that under the employed conditions the fast to slow conversion of chronically stimulated fast-twitch rabbit muscle is not exclusively caused by adult fiber transformation, but results in part from the substitution of fast-twitch glycolytic fibers with newly formed fibers that have a high oxidative profile.

Key words

Muscle, striated, skeletal Stimulation, chronic Degeneration Regeneration Myosin, embryonic Rabbit 

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Alfred Maier
    • 1
  • Brigitte Gambke
    • 1
  • Dirk Pette
    • 1
  1. 1.Fakultät für BiologieUniversität KonstanzKonstanzBundesrepublik Deutschland
  2. 2.Fakultät f. Biologie der Universität KonstanzKonstanz 1Federal Republic of Germany
  3. 3.Department of Cell Biology and AnatomyUniversity of Alabama at BirminghamBirminghamUSA