Human Genetics

, Volume 84, Issue 6, pp 571–573

Screening for steroid sulfatase (STS) gene deletions by multiplex DNA amplification

Authors

  • Andrea Ballabio
    • Howard Hughes Medical Institute, Baylor College of Medicine
    • Institute for Molecular Genetics, Baylor College of Medicine
  • Joel E. Ranier
    • Institute for Molecular Genetics, Baylor College of Medicine
  • Jeffrey S. Chamberlain
    • Institute for Molecular Genetics, Baylor College of Medicine
  • Massimo Zollo
    • International Institute of Genetics and Biophysics, CNR
  • C. Thomas Caskey
    • Howard Hughes Medical Institute, Baylor College of Medicine
    • Institute for Molecular Genetics, Baylor College of Medicine
Short Communications

DOI: 10.1007/BF00210812

Cite this article as:
Ballabio, A., Ranier, J.E., Chamberlain, J.S. et al. Hum Genet (1990) 84: 571. doi:10.1007/BF00210812

Summary

Deletions are the most common molecular defect in steroid sulfatase (STS) deficiency. We describe the application of multiplex DNA amplification, by polymerase chain reaction, for deletion screening in patients with STS deficiency (STS-PCR). Genomic DNA from 38 unrelated patients was amplified using two sets of primers, corresponding to the 5′ and the 3′ ends of the STS gene. The analysis of the amplified products was always consistent with the results obtained by Southern analysis. This method represents a sensitive fast non-radioactive test for detecting STS gene deletions.

Copyright information

© Springer-Verlag 1990