Skip to main content
Log in

Characterization of relic DNA from barley genome

Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

High-molecular-weight “relic” DNA fraction can be electrophoretically separated from the bulk of barley DNA digested with different restriction enzymes. We have cloned and analyzed a population of relic DNA fragments. The majority of AluI-relic DNA clones contained barley simple sequence satellite DNA and other families of repetitive DNA. One of these families, designated HvRT, has been analyzed in detail. This family is composed of tandemly arranged 118-bp monomers and is present in 7 × 105 copies in the barley genome. Clones representing the HvRT family were sequenced. HvRT repeats were found to contain high levels of methylated cytosine. The HvRT family was found in the genomes of H. vulgare, H. leporinum, H. murinum, H. jubatum, but not in H. marinum, H. geniculatum, and wheat. Different barley species and cultivars show restriction fragment length polymorphism with the HvRT probe. Chromosome-specific subfamilies of HvRT were found to be present on different barley chromosomes, providing the possibility of using the HvRT probe as a chromosome specific marker. HvRT fragments up to 810 kbp in length were resolved by pulsed field gel electrophoresis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Ananiev EV, Ryzik MV, Sonina NV, Golovkin MV, Shipkova NI (1988) Mobile genetic elements of cereal genomes. Biochem Physiol Pflanz 183:129–141

    Google Scholar 

  • Anderson S (1981) Shotgun DNA sequencing using cloned DNaseI-generated fragments. Nucleic Acids Res 9:3015–3027

    Google Scholar 

  • Barnes SM, James AM, Jamieson G (1985) The organization, nucleotide sequence, and chromosomal distribution of a satellite DNA from Allium cepa. Chromosoma 92:185–192

    Google Scholar 

  • Bedbrook JR, Jones J, O'Dell M, Thompson RD, Flavell RB (1980) A molecular description of telomeric heterochromatin in Secale species. Cell 19:545–560

    Google Scholar 

  • Benslimane AA, Dron M, Hartmann C, Rode A (1986) Small tandemly repeated DNA sequences of higher plants likely originate from a tRNA gene ancestor. Nucleic Acids Res 14:8111–8119

    Google Scholar 

  • Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version 2. Plant Mol Biol Rep 1:19–21

    Google Scholar 

  • Dennis ES, Peacock WJ (1984) Knob heterochromatin homology in maize and its relatives. J Mol Evol 20:341–350

    Google Scholar 

  • Dennis ES, Gerlach WL, Peacock WJ (1980) Identical polypyrimidine-polypurine satellite DNA in wheat and barley. Heredity 44:349–366

    Google Scholar 

  • Flavell RB (1982) Sequence amplification, deletion, and rearrangement: major sources of speciation during species divergence. In: Dover GA, Flavell RB (eds) Genome evolution. Academic Press, London New York, pp 301–323

    Google Scholar 

  • Grellet F, Delcasso D, Panabieres F, Delseny M (1986) Organization and evolution of a higher plant alphoid-like satellite DNA sequence. J Mol Biol 187:495–507

    Google Scholar 

  • Grosveld FG, Lund T, Murray EJ, Mellor AL, Dahl HHM, Flavell RA (1982) The construction of cosmid libraries which can be used to transform eukaryotic cells. Nucleic Acids Res 10:6715–6732

    Google Scholar 

  • Gruenbaum Y, Naveh-Many T, Cedar H, Razin A (1981) Sequence specificity of methylation in higher plant DNA. Nature 292:860–862

    Google Scholar 

  • Horz W, Zachau HG (1977) Characterization of distinct segments in mouse satellite DNA by restriction nucleases. Eur J Biochem 73:383–392

    Google Scholar 

  • Islam AKMR, Shepherd KW, Sparrow DHB (1981) Isolation and characterization of euplasmic wheat-barley chromosome addition lines. Heredity 46:161–174

    Google Scholar 

  • Jorgensen RB (1986) Relationships in the barley genus (Hordeum): an electrophoretic examination of proteins. Hereditas 104:273–291

    Google Scholar 

  • Junghans H, Metzlaff M (1988) Genome-specific, highly repeated sequences of Hordeum vulgare: cloning, sequencing and squash dot test. Theor Appl Genet 76:728–732

    Google Scholar 

  • Kato A, Yakura K, Tanifuji S (1984) Sequence analysis of Vicia faba repeated DNA, the FokI repeat element. Nucleic Acids Res 12:6415–6426

    Google Scholar 

  • Khvyrleva ZD, Gazumian AK, Ananiev EV (1988) Organization and nucelotide sequence of genes coding for 5s rRNA in barley (Hordeum vulgare). Genetika 24:1830–1840

    Google Scholar 

  • Kieny MP, Lathe R, Lecocq JP (1983) New versatile cloning and sequencing vectors based on bacteriophage M13. Gene 26:91–99

    Google Scholar 

  • Kieser T (1984) Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli. Plasmid 12:19–36

    Google Scholar 

  • Leclerc RF, Siegel A (1987) Characterization of repetitive elements in several Cucurbita species. Plant Mol Biol 8:497–507

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY

    Google Scholar 

  • Martinez-Zapater JM, Estelle MA, Somerville CR (1986) A highly repeated DNA sequence in Arabidopsis thaliana. Mol Gen Genet 204:417–423

    Google Scholar 

  • Metzlaff M, Troebner W, Baldauf F, Schlegel R, Cullum J (1986) Wheat-specific repetitive DNA sequences — construction and characterization of four different genomic clones. Theor Appl Genet 72:207–210

    Google Scholar 

  • Reed KC, Mann DA (1985) Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res 13:7207–7221

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Schwartz DC, Cantor CR (1984) Separation of yeast chromosome-sized DNAs by pulsed field gel electrophoresis. Cell 37:67–75

    Google Scholar 

  • Willard HF, Waye JS (1987) Hierarchical order in chromosome specific human alpha satellite DNA. Trends Genet 3:192–198

    Google Scholar 

  • Wu T, Wu R (1987) A new rice repetitive DNA shows sequence homology to both 5s RNA and tRNA. Nucleic Acids Res 15:5913–5923

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by Yu. Gleba

Rights and permissions

Reprints and permissions

About this article

Cite this article

Belostotsky, D.A., Ananiev, E.V. Characterization of relic DNA from barley genome. Theoret. Appl. Genetics 80, 374–380 (1990). https://doi.org/10.1007/BF00210075

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00210075

Key words

Navigation