Mycorrhiza

, Volume 5, Issue 5, pp 313–319

A tripartite culture system for endomycorrhizal inoculation of micropropagated strawberry plantlets in vitro

  • Abdelmalek Elmeskaoui
  • Jean-Pierre Damont
  • Marie-Josée Poulin
  • Yves Piché
  • Yves Desjardins
Original Paper

DOI: 10.1007/BF00207403

Cite this article as:
Elmeskaoui, A., Damont, JP., Poulin, MJ. et al. Mycorrhiza (1995) 5: 313. doi:10.1007/BF00207403

Abstract

The objective of the current investigation was to develop a reliable method to obtain vesicular arbuscular mycorrhizae (VAM) in micropropagated plantlets and to determine their influence on growth. An in vitro system for culturing the VA mycorrhizal fungus Glomus intraradices with Ri T-DNA-transformed carrot roots or nontransformed tomato roots was used in this study as a potential active source of inoculum for the colonization of micropropagated plantlets. After root induction, micropropagated plantlets grown on cellulose plugs (sorbarod) were placed in contact with the primary mycorrhizae in growth chambers enriched with 5000 ppm CO2 and fed with a minimal medium. After 20 days of tripartite culture, all plantlets placed in contact with the primary symbiosis were colonized by the VAM fungus. As inoculum source, 30-day-old VA mycorrhizal transformed carrot roots had a substantially higher infection potential than 5-, 10-or 20-day-old VAM. Colonized plantlets had more extensive root systems and better shoot growth than control plants. The VAM symbiosis reduced the plantlet osmotic potential. This response may be a useful pre-adaptation for plantlets during transfer to the acclimatization stage.

Key words

Micropropagation Glomus intraradices Strawberry Vesicular-arbuscular mycorrhizae (VAM) 

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Abdelmalek Elmeskaoui
    • 1
  • Jean-Pierre Damont
    • 2
  • Marie-Josée Poulin
    • 2
  • Yves Piché
    • 2
  • Yves Desjardins
    • 1
  1. 1.Centre de Recherche en Horticulture, Faculté des Sciences de l'Agriculture et de l'Alimentation,Université LavaiQuébecCanada
  2. 2.Centre de Recherche en Biologie Forestière Faculté de Foresterie et de Géomatique, Université LavalQuébecCanada

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