Applied Microbiology and Biotechnology

, Volume 39, Issue 4, pp 553–557

A combined polymerase chain reaction-colour development hybridization assay in a microtitre format for the detection of Clostridium spp.

  • Iván Galindo
  • Rafael Rangel-Aldao
  • José Luis Ramírez
Applied Genetics and Regulation

DOI: 10.1007/BF00205050

Cite this article as:
Galindo, I., Rangel-Aldao, R. & Ramírez, J.L. Appl Microbiol Biotechnol (1993) 39: 553. doi:10.1007/BF00205050

Abstract

We have developed a rapid and sensitive assay for the detection of clostridial cells or spores in liquid food samples. The method recognizes Clostridium 16S (small ribosomal subunit RNA) rDNA (ribosomal DNA) sequences by a polymerase chain reaction-digoxigenin-labelling protocol, coupled to a capture oligonucleotide immobilized on a microtitre plate. The positive results are revealed by means of a colour reaction. In 6 h of non-intensive labour, we can detect as few as two to five clostridial cells or spores in experimentally contaminated soft drinks.

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Iván Galindo
    • 1
    • 3
  • Rafael Rangel-Aldao
    • 1
    • 2
  • José Luis Ramírez
    • 3
  1. 1.Gerencia Nacional de BiotecnologíaCervecería Polar, C.A.CaracasVenezuela
  2. 2.Universidad Simón BolívarCaracasVenezuela
  3. 3.Laboratorio de Genética Molecular, Facultad de CienciasUniversidad Central de VenezuelaCaracasVenezuela

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