A combined polymerase chain reaction-colour development hybridization assay in a microtitre format for the detection of Clostridium spp.
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- Galindo, I., Rangel-Aldao, R. & Ramírez, J.L. Appl Microbiol Biotechnol (1993) 39: 553. doi:10.1007/BF00205050
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We have developed a rapid and sensitive assay for the detection of clostridial cells or spores in liquid food samples. The method recognizes Clostridium 16S (small ribosomal subunit RNA) rDNA (ribosomal DNA) sequences by a polymerase chain reaction-digoxigenin-labelling protocol, coupled to a capture oligonucleotide immobilized on a microtitre plate. The positive results are revealed by means of a colour reaction. In 6 h of non-intensive labour, we can detect as few as two to five clostridial cells or spores in experimentally contaminated soft drinks.