Cell and Tissue Research

, Volume 227, Issue 3, pp 563–568

A cell line (HBL-100) established from human breast milk

  • Edwin V. Gaffney

DOI: 10.1007/BF00204786

Cite this article as:
Gaffney, E.V. Cell Tissue Res. (1982) 227: 563. doi:10.1007/BF00204786


A continuous cell line (HBL-100) was obtained from primary cultures of cells derived from an early lactation sample of human milk. There was no evidence of a breast lesion in the milk donor. Karyotype analysis showed that all metaphases contained human chromosomes including a large acrocentric marker chromosome. Both desmosomes and cytoplasmic tonofibrils were observed during early passage. HBL-100 cells exhibited several characteristics of transformation including the ability to form colonies in soft agar, an aneuploid chromosome complement, and continuous growth.

Key words

MilkHumanCell culturesTransformation

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Edwin V. Gaffney
    • 1
  1. 1.Microbiology Program, The Pennsylvania State UniversityUniversity ParkUSA
  2. 2.306 South Frear Building, The Pennsylvania State UniversityUniversity ParkUSA