, Volume 5, Issue 2, pp 89-97

Formation and growth of the ectomycorrhiza of Cantharellus cibarius

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Abstract

New data on the physiology of Cantharellus cibarius mycorrhiza formation has resulted in a new aseptic routine method for in vitro formation. The advances are short formation time, healthy plants and reliable colonization. A high glucose demand and a good gas exchange with additional carbon dioxide are important factors in the mycorrhiza formation. Mycorrhiza was observed after 8 weeks, but strong colonization occurred after 10–12 weeks, when mycorrhiza was established to the depth of 5 cm. A C. cibarius strain connected to Picea abies in nature successfully colonized Pinus sylvestris in vitro, but not Betula pendula. Mycorrhizal plants have been successfully transferred to unsterile environments in greenhouses. The mycorrhizae continued to colonize new roots and the unsterile peat soil for 10 months. However, C. cibarius mycorrhiza is highly sensitive to flooding. With PCR and RFLP, fruit bodies, isolated mycelia and artificially formed mycorrhizae have been compared to prove that C. cibarius was used. Climatic changes did not induce primordia formation but factors behind fruit body formation are discussed.