, Volume 187, Issue 2, pp 171-175

Evidence for a geranyl-diphosphate synthase located within the plastids of Vitis vinifera L. cultivated in vitro

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Intact plastids from cell suspensions of Vitis vinifera L. cv. Muscat de Frontignan, free of detectable contamination by other particles as judged by the distribution of organelle-specific marker enzymes and by electron microscopy, exhibit geranyl-diphosphate synthase activity (EC This synthase activity remains stable after tryptic digestion of unlysed organelles and is enhanced by plastid disruption. We conclude that the enzyme is located within the organelle. The possibility of an isopentenyl diphosphate/dimethylallyl diphosphate translocating system which would play a major role in the regulation of monoterpene metabolism is discussed.

The authors gratefully acknowledge the help of A. Hamilton (University of Nottingham, UK) and Dr. M. Bouzayen (Ecole Nationale Supérieure Agronomique de Toulouse, France) in carefully reading this manuscript. This paper represents a part of the research submitted by E. Soler in partial fulfillment of the requirement for the Doctorate Degree. This work was supported by the Ministère de l'Education Nationale, de la Jeunesse et des Sports (Grant 88–1994).