Human Genetics

, Volume 87, Issue 6, pp 642–648

The human transmembrane secretory component (poly-Ig receptor): molecular cloning, restriction fragment length polymorphism and chromosomal sublocalization

Authors

  • P. Krajči
    • Laboratory for Immunohistochemistry and Immunopathology (LIIPAT)Institute of Pathology, University of Oslo The National Hospital
  • K. H. Grzeschik
    • Institut für Humangenetik der Universität
  • A. H. M. Geurts van Kessel
    • Department of Human GeneticsUniversity Hospital Nijmegen
  • B. Olaisen
    • Institute for Forensic Medicine, University of Oslo, The National Hospital
  • P. Brandtzaeg
    • Laboratory for Immunohistochemistry and Immunopathology (LIIPAT)Institute of Pathology, University of Oslo The National Hospital
Original Investigations

DOI: 10.1007/BF00201717

Cite this article as:
Krajči, P., Grzeschik, K.H., van Kessel, A.H.M.G. et al. Hum Genet (1991) 87: 642. doi:10.1007/BF00201717

Summary

The human transmembrane secretory component (SC) mediates glandular translocation of polymeric IgA and IgM into exocrine secretions. A 2898-bp cDNA clone, encoding the entire sequence of the human transmembrane SC, was isolated from a colonic adenocarcinoma cell line cDNA library. The deduced amino-acid sequence had a length of 764 residues and showed an overall similarity of 56% and 64% with the rabbit and rat counterpart, respectively. A restriction fragment length polymorphism (RFLP) was found with PvuII, revealing a two-alle RFLP with an autosomal codominant inheritance pattern and allele frequencies of 0.65 and 0.35. Southern blot analysis of human-rodent somatic hybrid panels, including hybrids with translocation chromosomes carrying different parts of chromosome 1, assigned the SC gene to 1q31-q42, thus confirming a previously reported provisional assignment.

Copyright information

© Springer-Verlag 1991