Original Investigations

Human Genetics

, Volume 87, Issue 6, pp 642-648

First online:

The human transmembrane secretory component (poly-Ig receptor): molecular cloning, restriction fragment length polymorphism and chromosomal sublocalization

  • P. KrajčiAffiliated withLaboratory for Immunohistochemistry and Immunopathology (LIIPAT), Institute of Pathology, University of Oslo The National Hospital
  • , K. H. GrzeschikAffiliated withInstitut für Humangenetik der Universität
  • , A. H. M. Geurts van KesselAffiliated withDepartment of Human Genetics, University Hospital Nijmegen
  • , B. OlaisenAffiliated withInstitute for Forensic Medicine, University of Oslo, The National Hospital
  • , P. BrandtzaegAffiliated withLaboratory for Immunohistochemistry and Immunopathology (LIIPAT), Institute of Pathology, University of Oslo The National Hospital

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Summary

The human transmembrane secretory component (SC) mediates glandular translocation of polymeric IgA and IgM into exocrine secretions. A 2898-bp cDNA clone, encoding the entire sequence of the human transmembrane SC, was isolated from a colonic adenocarcinoma cell line cDNA library. The deduced amino-acid sequence had a length of 764 residues and showed an overall similarity of 56% and 64% with the rabbit and rat counterpart, respectively. A restriction fragment length polymorphism (RFLP) was found with PvuII, revealing a two-alle RFLP with an autosomal codominant inheritance pattern and allele frequencies of 0.65 and 0.35. Southern blot analysis of human-rodent somatic hybrid panels, including hybrids with translocation chromosomes carrying different parts of chromosome 1, assigned the SC gene to 1q31-q42, thus confirming a previously reported provisional assignment.