Planta

, Volume 182, Issue 2, pp 194–198

Extracellular deamination of l-amino acids by Chlamydomonas reinhardtii cells

  • J. Muñoz-Blanco
  • J. Hidalgo-Martínez
  • J. Cárdenas
Article

DOI: 10.1007/BF00197110

Cite this article as:
Muñoz-Blanco, J., Hidalgo-Martínez, J. & Cárdenas, J. Planta (1990) 182: 194. doi:10.1007/BF00197110

Abstract

When grown in the light and in a Tris-acetate phosphate medium, cells of Chlamydomonas reinhardtii Dang. can use the following l-amino acids as a sole nitrogen source: asparagine, glutamine, arginine, lysine, alanine, valine, leucine, isoleucine, serine, methionine, histidine, and phenylalanine, whereas, in the absence of acetate, the cells only used l-arginine. The utilization system in the acetate medium consisted of an extracellular deaminating activity induced by l-amino acids; it took between 10 to 30 h before the system appeared in cells previously grown with ammonium. This deaminase activity was nonspecific, required an organic carbon source for its de-novo synthesis, and was sensitive to high ammonium concentration and light deprivation.

Key words

L-amino acids (utilization) Chlamydomonas Deaminase (extracellular) 

Abbreviations

HPLC

high-performance liquid chromatography

TAP

Tris-acetate-phosphate

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • J. Muñoz-Blanco
    • 1
  • J. Hidalgo-Martínez
    • 1
  • J. Cárdenas
    • 1
  1. 1.Departamento de Bioquímica y Biología Molecular y Fisiología, Facultad de CienciasUniversidad de CórdobaCórdobaSpain

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